Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

YPD/YPED Plates are used for the growth of Saccharomyces cerevisiae (yeast). Applications A solid medium for the propagation of yeast.

Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and…

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization. The concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X. …

TRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X). Applications Tris Glycine Transfer Buffer, at a 1X concentration with the addition of 20% methanol, is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane – i.e. Western blotting or…

LB Broth, Lennox is a ready-to-use microbial growth medium used for the cultivation of E. coli. Low (5 g/L) salt formulation Autoclave sterilized Applications LB Broth is a low-salt formulation in comparison to its counterpart LB Broth, Bertani Miller. Lennox is also nutrient rich…

Sodium Acetate, 3M pH 5.2 is a common molecular biology reagent. All production lots are tested to ensure that they are free of Dnase, Rnase & Proteases. It is often used in buffer systems to maintain the pH of a given solution. Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the…

Amphotericin B, Penicilllin, Stretomycin Mix is useful for reducing the chance of microbial contamination. Convenient mixture of the antibiotics and antifungal agent in one bottle. 100X concentration, just dilute 1:100 in the culture medium Recommended for use in cell culture applications at…

MEM NEAA contains the same non-essential amino acids found in the standard MEM, at a strength of 100. MEM NEAA 100X can be used to enhance cell growth and prolong the viability of cells in culture. 100X of Non-essential amino acids MEM NEAA 100X is commonly used as a supplement to minimal…

Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or…

Compared to other basal media, Ham’s F-12 contains a wider variety of components, and no proteins or growth factors. Without hypoxanthine and L-glutamine Ham’s F-12 uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH. …

Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications…

M9 CA Media contains the essentials for bacterial species to grow. Glucose as a carbon source and casamino acids as a nitrogen source. Important components of M9 CA medium are the carbon source from glucose, a nitrogen source from ammonia chloride, and electrolytes from sodium, chloride,…

DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting. Contains: 0.5 M NaOH and 1.5 M NaCl Filtered through a 0.22 µm membrane Dnase tested …

YES Agar Plates are for use of propagation and maintenance of yeasts for use in molecular biology Applications YES Agar Plates are for the growth of yeast.

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…

LB Agar Plates are used for the growth and maintenance of E. coli strains used in molecular microbiology procedures. Without any antibiotics Applications The following variations of LB Agar Plates are nutritionally rich media designed for growth of pure cultures of recombinant strains.…

Nuclease and Endotoxin-Free, Sterile DEPC-treated water is certified nuclease-free and supplied in four bottles containing 1 L each to ensure sterility and inactivation of DEPC. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity, protease. …

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

Tris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L Sodium Bicarbonate Without HEPES buffer Applications E199 has broad species applicability, particularly for cultivation of…

Designed for cell culture without CO2. Without Sodium Bicarbonate With Phenol Red Without L-glutamine Applications L-15 has been used for the culture of HEp-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations EMEM Enriched is regular EMEM supplemented with 2X penicillin, streptomycin and L-glutamine.

A hybridization buffer specifically formulated for use with nylon membranes. It does not contain Denhardt’s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. Formulated for Nylon membrane Filtered through a 0.22 µm membrane DNase, RNase, and Protease…

Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We…

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E. Without L-glutamine Applications This rich medium was used to culture the hepatocytes derived from an enrichment procedure also developed by Dr. Williams and his colleagues in…

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. Ready-to-use solution Applications Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and…

Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi. 100X concentration. Just dilute 1:100 to get the final working concentration of 2.50 µg/mL Antifungal agent Amphotericin B prevents the growth of fungi by causing an increase…