Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield. Autoclave sterilized Ready to use Applications …

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol Red Applications DPBS can be used to provide a…

Calcium Chloride 2 M is useful in the preparation of competent E.coli and transformation of bacteria. Preparation of competent E.coli Transformation of bacteria 2 Molar concentration Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease tested Applications Calcium chloride is…

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators…

Normal Saline’s isotonic solution is used as a general laboratory diluent. With 9 g/L sodium chloride For non clinical use Applications Normal Saline can be used in the following microbiology applications: solvent, washing agent for cells/bacteria/specimen, buffer in aseptic techniques…

This is a special formulation of loading solution for protein gel electrophoresis. Special formulation Applications SDS-PAGE Western Blotting Analysis of DNA samples with high amounts of DNA binding proteins Kinetic experiments DNA agarose gel analysis after DNA restriction digestions,…

QBSF-60 is a proprietary serum-free medium that supports the expansion of human hematopoietic stem/progenitor cells. QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to…

Trypticase Soy Agar with 5% Sheep Blood is used for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. Applications Grow a wide variety of fastidious organisms on TSA with Sheep Blood, a general purpose non-selective…

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Reduce nonspecific binding Applications Quality Biological’s Blocking Solution is prepared from non-fat dried milk in PBS containing sodium azide 0.02% (w/v) as a…

Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications…

YPD/YPED Plates are used for the growth of Saccharomyces cerevisiae (yeast). Applications A solid medium for the propagation of yeast.

Applications Standard, unstabilized solutions of L-glutamine spontaneously break down over time to release cytotoxic amines. Glutamine XL is a stabilized solution that prevents degradation and cytotoxicity, with the benefit of a long shelf life. The incorporated L-alanine protects the unstable…

Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well. 5X concentrated protein gel loading buffer Applications Simply load the gel sample wells and visualize progress of the electrophoresis run by…

Synthetic Dextrose (SD) Medium is a defined minimal yeast medium. Applications SD is a synthetic minimal medium containing salts, trace elements, vitamins, a nitrogen source (Bacto-yeast nitrogen base without amino acids), and glucose.

Sodium Chloride, 0.85% is a saline solution at 0.85% prepared with water used for cell culture. Made with cell culture grade water The sodium chloride is a source of chlorine and sodium necessary to the production of components like sodium carbonate, sodium hydroxide or chlorate ions. …

DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. …

Sodium acetate is a widely used reagent in molecular biology applications. It is used as a buffer in conjunction with acetic acid, in the buffering range of pH 3.6 – 5.6. Applications Sodium acetate is used in the purification and precipitation of nucleic acids, protein crystallization,…

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989). The purpose of this reagent is to insure that the RNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. Contains two dyes, bromphenol blue…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without Phenol Red With Glucose and L-glutamine Applications EMEM (Eagle’s Minimum Essential Medium) has…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…

Tris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris…

Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi. 100X concentration. Just dilute 1:100 to get the final working concentration of 2.50 µg/mL Antifungal agent Amphotericin B prevents the growth of fungi by causing an increase…

DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting. Contains: 0.5 M NaOH and 1.5 M NaCl Filtered through a 0.22 µm membrane Dnase tested …

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H. Used with SOC medium Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure,…

Sodium Acetate, 3M pH 5.2 is a common molecular biology reagent. All production lots are tested to ensure that they are free of Dnase, Rnase & Proteases. It is often used in buffer systems to maintain the pH of a given solution. Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With High Glucose Without L-Glutamine Without Phenol red Without HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…