Percoll® is a medium for density gradient centrifugation of cells, viruses and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which renders the material completely non-toxic and ideal for use with biological materials. Centrifugation…

Terrific Broth II is a nutritionally rich medium for the growth of E. coli cells containing yeast extract and tryptone in elevated concentrations as well as sodium chloride and vitamins for a luxurious growth. Molecular Biology Certified Proprietary formulation that is shipped as a single…

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also…

Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Agar Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Agar formulation and thoroughly blended for a…

Schizosaccharomyces pombe media certified to be thiamine-free for nmt1 promoter studies. Molecular Biology Certified Contains 5 g Yeast Extract, 30 g Dextrose, 1X Supplements (50 mg each ADE, HIS, LEU, LYS, URA). Small quantities of biological buffers have been added for proper pH…

DOB 2% Galactose, 1% Raffinose allows for preparation of a custom synthetic defined yeast media and includes a complete yeast nitrogen base (YNB) composed of buffering salts, vitamins and trace elements, with Galactose and Raffinose as the carbon source and Agar.

Quadruple dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Molecular Biology Certified For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media Select for…

Sodium pyruvate is utilized as a component in culture broth and media. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably…

X-Gal (5-Bromo-4-Chloro-3-Indolyl-ß-D-galactopyranoside) is a β-galactosidase substrate. In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate. Identification and selection of galactosidase producing bacteria Detects activity of…

CSM-MET is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Dropout supplement for virtually all strains of S. cervisiae Add DOB or DOBA Base Medium for vigorous cell growth Selects for suxotrophic requirements and…

ITS Premix stimulates cell proliferation while decreasing substantially the serum requirements for culture of many cell types. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 µg/ml sodium selenite. Prepared in Earle's balanced Salt…

SC Amino Acid Mixture, also known as Hopkins Mixture, is a Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Molecular Biology Certified For use with DOB or YNB with approoriate carbon and nitrogen sources Used to formulate selected Synthetic…

L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. Molecular Biology Certified Supports luxurious growth of E. coli Tryptone and yeast extract supply essential growth factors Contains essential electrolytes for…

Calcium and magnesium support cell adhesion. Hanks' Balanced Salts (Modified), without Magnesium and Calcium, is used to minimize cell attachment or aggregation.

CSM-Leu is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Molecular Biology Certified Add DOB or DOBA base medium for vigorous cell growth Select for auxotrophic requirements and transformants Media Preparation:…

LB-Medium, Lennox Powder is Luria-Bertani, Rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium. Molecular Biology Certified Widely used in molecular biology Supports a luxurious groth of E. coli cells Tryptone and yeast…

Freund's complete adjuvant is a mixture containing mineral oil, Arlacel A, and heat-killed Mycobacterium butyricum: Freund's Complete Adjuvant. Components are thoroughly mixed in controlled proportions and autoclaved prior to being vialed. Freund's Adjuvants may be used to produce water-in-oil…

Yeast extract powder is a vacuum dried autolyzed yeast extract produced from 100% Baker's yeast. It is a concentrated source of soluble protein components, carbohydrates, and B Complex vitamins natural to yeast. Yeast extract is used as a bacteriological media component for a variety of…

YPD AGAR is a nutritionally rich medium for the growth of S. cerevisiae, containing dextrose, peptone and yeast extract and agar. Molecular Biology Certified Dextrose energy source Yeast extract and peptone provide carbon, nitrogen, minerals, vitamins, trace ingredients and other…

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the…

Double dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Molecular Biology Certified For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media Select for yeast…

LB medium is the most widely used medium for the growth of bacteria. Our encapsulated format eliminates the waste, inaccuracies and mess associated with weighing out bulk powder and offers increased convenience and ease of use.

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to…

Cell Cryopreservation Medium with 10% DMSO is a balanced, protein free, ready-to-use cryopreservation solution with 10% USP DMSO (Cryopres™). It does not require any dilution or further processing and can be used for cells cultured in serum free medium or with serum containing…

Sterile 80% Glycerol solution offered is less viscous and more convenient to pipette than 100% glycerol. Used in sample preparation and gel formation for polyacrylamide gel electrophoresis Aid in casting gradient gels Protein stabilizer and storage buffer component Bacteria and…

Triple dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Molecular Biology Certified For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media Select for yeast…

LB broth is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in a variety of formats for convenience and ease of use. Typically contain 10 g of tryptone and 5 g of yeast extract per liter, and vary in salt

Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations. Molecular Biology Certified For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom…

Murashige and Skoog Medium is widely used plant tissue culture growth medium. It has been proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the…

YNB is a well defined composition of salts, vitamins and a nitrogen source for a vigorous growth of Saccharomyces cerevisiae. Molecular Biology Certified Component of synthetic defined yeast media Reagents are tested and chosen for their ability to promote optimum growth of S.…

DOBA is a complete yeast nitrogen base with a carbon source and agar. Molecular Biology Certified

Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Agar formulation and thoroughly blended for a uniform…