Preparation Method
Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Agar formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Applications
LB Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The ultimate in ease and convenience, LB Medium in a capsule form eliminates the waste, inaccuracies, and mess associated with weighing out bulk powder. The growth characteristics are the same as LB agar powder. LB Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Product Overview
LB medium capsules have the same high quality formulation as LB powder with the added advantage of being in a capsule form, eliminating the need for weighing and handling. The growth characteristics are the same as LB powder.
Product Description
LB medium capsules is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in an encapsulated format for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
Recommended Use
Add 25 capsules per liter of purified water and autoclave at 121°C for 15 minutes.