L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains.
Molecular Biology Certified
- Supports luxurious growth of E. coli
- Tryptone and yeast extract supply essential growth factors
- Contains essential electrolytes for transport and osmotic balance
Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Medium formulations. Chosen reagents are combined in the accurate proportions according to the LB formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid. The combinations were mixed to dissolve components and then autoclaved at 121°C for 15 minutes.