The LGC expression recovery medium with a capacity of 12 mL helps in the stabilization and transformation of protein expression clones in chemical laboratories and research facilities.

EZ-Tn5™ Transposase is a hyperactive Tn5 transposase for random insertion of an EZ-Tn5 Transposon into any cloned DNA in vitro or for preparing an EZ-Tn5 Transposome for random insertion of a EZ-Tn5 Transposon into the genomic DNA of living bacteria.

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of…

The NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers. Each box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.

The AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…

Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.

RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…

Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of…

AmpliScribe™ T7 High Yield Transcription Kits are specially formulated to produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions.

This reagent is designed for use with the MasterPure™ kit protocols only.

This reagent is designed for use with the MasterPure™ kit protocols only.

The MMLV High Performance Reverse Transcriptase produces full-length first-strand cDNA from total RNA preparations or purified poly(A+) RNA.

OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides.

The LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.

Phage T1-resistant electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.

The Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3´ end of any RNA. Poly(A) Polymerase is encoded by an E. coli gene that has been cloned into a plasmid and overexpressed in an E. coli strain.

MasterAmp™ Tth DNA Polymerase is a recombinant thermostable DNA polymerase for use in PCR. This enzyme has optimal DNA synthetic activity at temperatures above 70°C and can be used at temperatures up to 95°C.

RNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi

For use with the MasterAmp™ PCR Optimization Kits.

The NxSeq HT Dual Indexing Kit is required for the construction of DNA fragment libraries using the NxSeq UltraLow Kit, 96 Reactions. This kit provides the Universal Adaptor and Dual Indexing Primers required for the construction of 96 libraries with unique dual indices.

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.

M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3'′→5' proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

EpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity

Perfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…

Electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.

Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…

Chemically competent E. coli engineered to regulate the copy number of CopyControl Fosmid clones.

Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures