LGC, Biosearch Technologies

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LGC, Biosearch Technologies

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  • Ribonuclease R (RNase R) 250 U

    LGC, Biosearch Technologies

    Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3´′→5´ exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures.

  • RapiDxFire Thermostable Reverse Transcriptase

    LGC, Biosearch Technologies

    RapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…

  • Terminator™ 5´-Phosphate-Dependent Exonuclease is a processive 5´′→3´ exonuclease that digests RNA that has a 5´-monophosphate end. It does not digest RNA that has a 5´-triphosphate, 5´-cap or 5´-hydroxyl group.

  • E. cloni 10G ELITE Electrocomp Cells

    LGC, Biosearch Technologies

    Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…

  • EZ-TN5 KAN-2 TNP TRANSPOSOME KIT 10 rxns

    LGC, Biosearch Technologies

    This Transposome is a stable transposon-transposase complex that can be electroporated into living bacterial cells in order to generate a libray of random gene knockouts. Transposed bacteria are selected on kanamycin plates.

  • COPYCONTROL FOSMID AUTOINDUCTION SOLN 50 ml

    LGC, Biosearch Technologies

    The CopyControl Autoinduction Solution will induce CopyContol Fosmid clones, grown in the TransforMax EC300 E. coli, to high copy number without the need for sub-culturing.

  • MasterAmp 10X PCR Enhancer 10 ml

    LGC, Biosearch Technologies

    For use with the MasterAmp™ PCR Optimization Kits.

  • MMLV HIGH PERFORMANCE REV TRANS 25,000 U

    LGC, Biosearch Technologies

    The MMLV High Performance Reverse Transcriptase produces full-length first-strand cDNA from total RNA preparations or purified poly(A+) RNA.

  • MasterAmp 2X PCR PreMix

    LGC, Biosearch Technologies

    The MasterAmp™ PCR Optimization Kits offer simplified PCR optimization with a thermostable DNA polymerase of choice, PCR of difficult or high-GC templates and PCR amplifications up to 20 kb. For rapid, dependable optimization of PCR, we encourage you to try the FailSafe™ PCR System to…

  • QuickExtract DNA Extraction Solution

    LGC, Biosearch Technologies

    The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.

  • Arabinose Solution, 1000X 5x1mL

    LGC, Biosearch Technologies

  • Episcript Rnase H- Reverse Transcriptase

    LGC, Biosearch Technologies

    EpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity

  • Endura Chemically Competent Cells

    LGC, Biosearch Technologies

    Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…

  • ClearColi BL21(DE3) Electrocompetent Cells

    LGC, Biosearch Technologies

    ClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by…

  • FailSafe PCR System

    LGC, Biosearch Technologies

    The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time….We promise!

  • Uracil N-Glycosylase 1,000 U

    LGC, Biosearch Technologies

    Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate

  • EconoTaq PLUS

    LGC, Biosearch Technologies

    EconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR. More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify…

  • Riboguard Rnase Inhibitor

    LGC, Biosearch Technologies

    RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.

  • Rec J Exonuclease 250 U

    LGC, Biosearch Technologies

    Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

  • NxGen T4 DNA Ligase

    LGC, Biosearch Technologies

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

  • Proteinase K 2 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • Expresso Rhamnose Cloning & Exp Systems

    LGC, Biosearch Technologies

    The Expresso Rhamnose Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and expression of PCR-amplified genes using Expressioneering Technology. Expressioneering Technology uses in vivo homologous recombination to seamlessly clone PCR amplified…

  • BIOTIN-16-UTP 500 nmoles

    LGC, Biosearch Technologies

    Biotin-16-UTP is readily incorporated into RNA during an in vitro transcription reaction by RNA polymerases such as T7, T3, or SP6 RNA Polymerases

  • RapiDxFire qPCR 5X Master Mix GF

    LGC, Biosearch Technologies

    RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…

  • MPC Protein Precipitation Solution 500 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • TE Buffer 70 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • Tissue & Cell Lysis Solution 600 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • The Expresso SUMO Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and soluble expression of PCR-amplified genes. For proteins that form inclusion bodies or are difficult to express in soluble form, the pETite® N-His SUMO and pRham™…

  • AmpliScribe™ T7 High Yield Transcription Kits are specially formulated to produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions.

  • 2'-dNTP (All 4 Mixed) 10 µmole

    LGC, Biosearch Technologies

    Sterile, neutral pH solutions of dATP, dCTP, dGTP, and dTTP are available either as individual nucleotides or a mixture of all four nucleotides for use in DNA synthesis.

  • LavaLAMP™ RNA Component Kits

    LGC, Biosearch Technologies

    The LavaLAMP™ RNA Component Kit is a deconstruction of the LavaLAMP™ RNA Master Mix, and as such, when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the…

  • Copycutter Epi400

    LGC, Biosearch Technologies

    CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins

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