LGC, Biosearch Technologies

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LGC, Biosearch Technologies

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  • Ampligase 10X Reaction Buffer 5 ml

    LGC, Biosearch Technologies

    Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures

  • EconoTaq DNA Polymerase

    LGC, Biosearch Technologies

    EconoTaq’s low price is coupled with high quality and performance. QC specifications for EconoTaq are rigorous: Greater than 99% pure by SDS gel electrophoresis. No detectable DNA contamination as determined by PCR using generic primers. No detectable endonuclease (nicking)…

  • BigEasy v2.0 Lin Cloning Kits

    LGC, Biosearch Technologies

    The BigEasy Kit with the pJAZZ vector is ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb. Because the novel pJAZZ vector is maintained as a linear molecule, the ends of the vector can rotate freely. As a result, the vector…

  • Ampligase DNA Ligase

    LGC, Biosearch Technologies

    Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures

  • BIOTIN-16-UTP 500 nmoles

    LGC, Biosearch Technologies

    Biotin-16-UTP is readily incorporated into RNA during an in vitro transcription reaction by RNA polymerases such as T7, T3, or SP6 RNA Polymerases

  • COPYCUTTER INDUCTION SOLUTION 25 ml

    LGC, Biosearch Technologies

    Prepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.

  • 2'-dNTP (All 4 Mixed) 10 µmole

    LGC, Biosearch Technologies

    Sterile, neutral pH solutions of dATP, dCTP, dGTP, and dTTP are available either as individual nucleotides or a mixture of all four nucleotides for use in DNA synthesis.

  • RapiDxFire qPCR 5X Master Mix GF

    LGC, Biosearch Technologies

    RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…

  • MESSAGEBOOSTER CDNA SYNTHESIS KIT

    LGC, Biosearch Technologies

    The MessageBOOSTER™ cDNA Synthesis Kit for qPCR amplifies the mRNA from as little as 10 pg of RNA for greatly improveds qRT-PCR sensitivity, accuracy, and reproducibility of even low-abundance transcripts.

  • RNase-Free DNase I

    LGC, Biosearch Technologies

    RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.

  • EZ-TN5 TET-1 INSERTION KIT 10 rxns

    LGC, Biosearch Technologies

    The kit randomly inserts a tetracycline resistance (TetR) marker into any cloned DNA.

  • Ampliscribe T7-Flash Transcription Kits

    LGC, Biosearch Technologies

    The AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

  • Endura ElectroCompetent Cells

    LGC, Biosearch Technologies

    Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…

  • RapiDxFire Hot Start Taq DNA Polymerase

    LGC, Biosearch Technologies

    RapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst…

  • The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes.

  • Sterile Nuclease-Free Water 50 ml

    LGC, Biosearch Technologies

    Epicentre's Molecular Biology Grade Nuclease-Free Water is free of DNA exo- and endonuclease activities, and RNase activity.

  • NxSeq UltraLow DNA Library Kits

    LGC, Biosearch Technologies

    The NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.

  • The kit randomly inserts an E. coli origin of replication into genomic DNA of living bacteria for rescue cloning of the region of DNA flanking the insertion site.

  • NxGen phi29 DNA Polymerase

    LGC, Biosearch Technologies

    phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

  • SS320 (MC1061 F) Electrocompetent Cells

    LGC, Biosearch Technologies

    Perfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…

  • TYPEONE RESTRICTION INHIBITOR 100 ug

    LGC, Biosearch Technologies

    TypeOne™ Restriction Inhibitor can significantly increase transformation efficiencies in bacterial strains with type I R-M systems. TypeOne Inhibitor is readily electroporated into cells along with transforming DNA including EZ-Tn5 Transposomes.

  • Glucose Solution, 15% w/v 5x1.25mL

    LGC, Biosearch Technologies

  • Expresso Rhamnose Cloning & Exp Systems

    LGC, Biosearch Technologies

    The Expresso Rhamnose Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and expression of PCR-amplified genes using Expressioneering Technology. Expressioneering Technology uses in vivo homologous recombination to seamlessly clone PCR amplified…

  • The MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria.

  • LavaLAMP DNA Component Kits

    LGC, Biosearch Technologies

    The LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…

  • Sbeadex viral RNA Purification Kit

    LGC, Biosearch Technologies

    sbeadex viral RNA purification kits are magnetic-bead based kits are validated for research use only using AccuPlex™ reference material (SeraCare), replicating a real-life clinical sample as far as practically possible, and are compatible with Biosearch Technologies’ COVID-19 detection kit.…

  • CircLigase II ssDNA Ligase

    LGC, Biosearch Technologies

    CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group

  • FosmidMAX DNA Purification Kit

    LGC, Biosearch Technologies

    The FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA.

  • RNA 5' Polyphosphatase 200 U

    LGC, Biosearch Technologies

    RNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi

  • EZ-TN5 R6KORI/KAN-2 INSERTION KIT 10 rxns

    LGC, Biosearch Technologies

    The kit randomly inserts a KanR gene and a R6Ky origin of replication into any cloned DNA for rescuing plasmids not normally replicated in E. coli.

  • Hybridase Thermostable RNase H 500 U

    LGC, Biosearch Technologies

    Hybridase™ Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA.

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