The LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…

The LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which…

One tube each of ATP, CTP, GTP and UTP at 100 mM. Each is provided as a sterile, neutral solution.

This reagent is designed for use with the MasterPure™ kit protocols only.

The LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…

Prepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.

The LGC recovery medium is used in chemical laboratories and helps in the growth and fostering off competent cells thus allowing the highest possible transformation efficiency.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures

Exonuclease I digests ssDNA in a 3´′→5´ direction, but does not digest dsDNA. Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes.…

The DuraScribe® T7 Transcription Kit produces 2'-Fluorine-modified RNA transcripts—called DuraScript® RNA—that are completely resistant to RNase A. Ideal for SELEX RNA-aptamer selection procedures.

Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…

RNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C…

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.

EconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR. More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify…

Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.

The MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit is optimized for generating full-length first-strand cDNA from total RNA preparations or purified Poly(A)+ RNA.

The NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.

CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group

CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi.

The LavaLAMP™ RNA Component Kit is a deconstruction of the LavaLAMP™ RNA Master Mix, and as such, when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the…

CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins

EpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…

Perfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…

Bst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription…

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…

RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.

ClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by…

Perfect for antibody phage display or peptide phage display library creation. ER2738 Electrocompetent Cells are an amber suppressor strain (glnV) prepared as highly efficient (=2 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New…

BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 10^10 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells…