LGC, Biosearch Technologies Chemicals Reagents P
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Episcript Rnase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity
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Ampligase DNA Ligase
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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Plasmid-Safe ATP-Dependent DNase
LGC, Biosearch TechnologiesPlasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
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MC1061 F- Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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FailSafe PCR System
LGC, Biosearch TechnologiesThe FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time….We promise!
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Recovery Medium
LGC, Biosearch TechnologiesThe LGC recovery medium is used in chemical laboratories and helps in the growth and fostering off competent cells thus allowing the highest possible transformation efficiency.
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Expression Recovery Medium (Lactose Minus)
LGC, Biosearch TechnologiesThe LGC expression recovery medium with a capacity of 12 mL helps in the stabilization and transformation of protein expression clones in chemical laboratories and research facilities.
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TRANSFORMAX EPI300 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically competent E. coli engineered to regulate the copy number of CopyControl Fosmid clones.
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NxSeq AmpFREE Low DNA Library Kits
LGC, Biosearch TechnologiesThe NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours…
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OmniCleave Endonuclease 50,000 U
LGC, Biosearch TechnologiesOmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides.
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Ready-Lyse Lysozyme Solution
LGC, Biosearch TechnologiesReady-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
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E. cloni 10G SUPREME Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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RapiDxFire Hot Start Taq DNA Polymerase
LGC, Biosearch TechnologiesRapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst…
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T7 R&DNA Polymerase 5,000 U
LGC, Biosearch TechnologiesT7 R&DNA™ Polymerase is a recombinant mutant form of T7 RNA polymerase (Y639F mutant), in which Tyr-639 has been replaced by Phe.
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RIBONUCL-5'-TRIPHOSPHATE (NTP) SOL 25 umole ea
LGC, Biosearch TechnologiesOne tube each of ATP, CTP, GTP and UTP at 100 mM. Each is provided as a sterile, neutral solution.
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RNase I 1,000 U
LGC, Biosearch TechnologiesRNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C…
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LavaLAMP DNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…
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MasterPure Complete DNA & RNA Purification Kits
LGC, Biosearch TechnologiesThe MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
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Copycutter Epi400
LGC, Biosearch TechnologiesCopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins
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LavaLAMP DNA Component Kits
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…
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E. cloni 5-alpha Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…
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SS320 (MC1061 F) Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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OverExpress Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…
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Exo-Minus Klenow DNA Polymerase 1,000 U
LGC, Biosearch TechnologiesExo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´′→3´ and 3´′→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).
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Baseline-ZERO DNase 5,000 U
LGC, Biosearch TechnologiesBaseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
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COPYCUTTER INDUCTION SOLUTION 25 ml
LGC, Biosearch TechnologiesPrepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.
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MasterPure Gram Positive DNA Purification Kit 100 Purif.
LGC, Biosearch TechnologiesThe MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria.
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E. cloni EXPRESS BL21(DE3) Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…
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NxGen RNAse Inhibitor
LGC, Biosearch TechnologiesRNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.
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Ampligase 10X Reaction Buffer 5 ml
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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BAC-Opt Replicator v2.0 Electrocomp Cells
LGC, Biosearch TechnologiesBAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 10^10 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells…
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Exonuclease VII 250 U @ 10 U/µl
LGC, Biosearch TechnologiesExonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.