bioWORLD, NEW! Protein Expression
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Bovine Serum Albumin (BSA)
bioWORLDReagent grade, fatty acid free Moisture: < 1.0% Heavy metals: <0.5% Endotoxin: ≤0.1EU/mg pH (10% water): 6.5 - 7.5
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MagneZoom™, G (Protein G)
bioWORLDRecombinant protein G covalently linked to MagneZoom (paramagnetic beads). Protein G binds Rc region of mammalian immunoglobulin. Ideal for purification and fractionation of IgG subclasses that poorly bind protein A. Suitable for high throughput screening. Particle size: 2-5µm Ligand:…
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Aldehyde-terminated Magnetic Beads
bioWORLDSilica-coated uniform superparamagnetic beads, MagneZoom, with an aldehyde surface group. It is widely used to covalently couple ligands containing primary and secondary amino groups. This coupling method will provide an extremely stable linkage between the ligand and the matrix. Size: 2-5µm …
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Poly-L-Lysine-Coated Microplate
bioWORLDPoly-Lysine coated microplates High quality coated microplates. The Poly-Lysine coating of these 96-well microplates offers specific, high affinity binding to a variety of cell types.
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Ni-alginate macro and micro beads
bioWORLDBioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…
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Streptavidin Separopore® (Agarose)
bioWORLDApplications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…
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Carboxy-terminated Magnetic Beads
bioWORLDSilica-coated uniform super paramagnetic beads with surface carboxyl group. Proteins and nucleic acids can be covalently bound to these beads with the retention of biological activity. Size: 5-10µm. COOH terminated Binding: up to 20mg/ml. pH stability: 4-9 Supplied as 25mg/ml…
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Bradford Protein Assay Kit, 1 L
bioWORLDBradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
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To remove non-specific binding of proteins to magnetic bead surface and to achieve clean co-immunoprecipitation of your desired antibody or any other ligand binding. Use it with any magnetic beads experiment. Size: 2-5µm. Silica-coated magnetic beads v pH stability: 4-9 Supplied as…
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BBS/Glycerol/Fish Gelatin
bioWORLDBuffered fish gelatin 0.05% blocks, diluents and coating solutions are supplied as 10X concentrates. Sold as 2 x 100mL or 500mL. pH range: 8.0 (+/- 0.1) formulated in borate buffered physiologic saline buffer containing glycerol as a preservative
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Protein A coated microplates provide alternative methods for immobilizing antibodies for ELISA and other plate-based assay techniques. These plates are uniformly and stably coated with Protein A. They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of…
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High quality microplates coated with a high binding heparin solution. Heparin, Sodium salt is a low molecular weight heparin (LMWH) polymer that produces its major anticoagulant effect by activating antithrombin. This activation causes a conformational change in ATIII and allows for increased…
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Wheat germ -Triticum vulgaris- agglutinin (WGA) linked to MagneZoom (magnetic beads). For magnetic separation by binding specifically to cell particles, glycoconjugates and polysachharides containing N-acetylglucosamine residues. Size: 2-5µm. Ligand: Wheat germ agglutinin Ligand…
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The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an…
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Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…
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Protein with numerous biochemical applications including ELISAs, immunoblots, and immunohistochemistry Biotechnology Grade Heat shock isolation Appearance: white to off-white powder pH (5% water): 6.5-7.5 Heavy Metals (Pb): DNase: None detected RNase: None detected Protease: None…
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Protein-A Separopore® 4B
bioWORLDAffinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…
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BSA (Bovine serum albumin)
bioWORLD1mg/ml BSA solution in 0.85% Sodium Chloride and 0.01% Sodium azide.
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Goat anti-Human IgG Nano-activeGold™
bioWORLDNano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™ reagents consist of affinity-purified IgG antibodies, goat anti-human IgG covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron…
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Cellbuster-A™ and Cellbuster-B™ Kits
bioWORLDCellBuster-A Kit (Animal cell protein prep.) An efficient cell lysis medium for animal cells for preparation of total cellular proteins. Cell lysis facilitated by combination of proprietary detergent mixture. The kit contains enough reagents for 50 medium scale cultures. …
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ConA-MagneZoom Kit is designed for quick and efficient small scale purification by magnetic separation of macromolecules containing a-D-mannosyl or a-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides. The kit includes paramagnetic beads of average 5 µm in diameter covalently…
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Q-Cons™Rapid Protein Concentrators
bioWORLDQuick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume
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Haptoglobin from Human Plasma, 1 mg
bioWORLDFunctions by binding to hemoglobin. 98.0% (HPCE), powder, color off-white Occurs as 3 major phenotypes: type 1-1, type 2-1, type 2-2
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The bioLifeTM MUG ß-Galactosidase Assay Kit provides a highly sensitive fluorogenic subsatrate for detection of ß-galactosidase activity in tranfected cells. In the presence of ß-galactosidase the colorless 4-methylumbelliferyl B-D-galactopyranoside (MUG) substrate is hydrolyzed…
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Streptavidin Coated Plates are available in 96-well plate format, and pre-blocked with BSA Blocking Buffer. bio-world Biotin-Binding Plates are available in clear to be used with colorimetricdetection methods. Easy and gentle immobilization of biotin-containing conjugates…
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Carboxyl Coupling Kit, 10 mL
bioWORLDbio-World's Carboxy Coupling Kit consists of amino-terminated Separopore® 4B-CL (4% crosslinked agarose beads). This kit is suitable for covalent immobilization of peptides or other carboxyl-containing (–COOH) molecules for use in affinity purification procedures. It includes carboxyl Coupling…
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Bradford Reagent, Ready to Use
bioWORLDBradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
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Biotin Coated Plates can be used as the basis for a variety of biomolecular assay techniques requiring the immobilization of probes that are conjugated with NeutrAvidin Protein, streptavidin, avidin or other biotin-binding proteins. Specifications: Biotin group accessible for binding…
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Isopropyl-ß-D-Thiogalactopyranoside. An inducer of ß-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. Biotechnology…
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MagneZoom™A Protein-A Magnetic Beads
bioWORLDRecombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants. Particle size: 2-5µm Ligand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beads pH stability: 4-9 …
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bioWorld's Carboxylated 96-well Microplates contains a COOH-coated surface to which the amino group present in any molecules, such as peptides or proteins, binds to through formation of amide bonds by the action of carbodiimide. Applications: Coupling molecules, having an amino group, to…
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Nickel-Coated Microplates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing…