bioWORLD, NEW! Protein Expression
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Bradford Reagent, Ready to Use
bioWORLDBradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
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Protein with numerous biochemical applications including ELISAs, immunoblots, and immunohistochemistry Biotechnology Grade Heat shock isolation Appearance: white to off-white powder pH (5% water): 6.5-7.5 Heavy Metals (Pb): DNase: None detected RNase: None detected Protease: None…
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Streptavidin Separopore® (Agarose)
bioWORLDApplications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…
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Isopropyl-ß-D-Thiogalactopyranoside. An inducer of ß-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. Biotechnology…
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Aldehyde-terminated Magnetic Beads
bioWORLDSilica-coated uniform superparamagnetic beads, MagneZoom, with an aldehyde surface group. It is widely used to covalently couple ligands containing primary and secondary amino groups. This coupling method will provide an extremely stable linkage between the ligand and the matrix. Size: 2-5µm …
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The bioLifeTM MUG ß-Galactosidase Assay Kit provides a highly sensitive fluorogenic subsatrate for detection of ß-galactosidase activity in tranfected cells. In the presence of ß-galactosidase the colorless 4-methylumbelliferyl B-D-galactopyranoside (MUG) substrate is hydrolyzed…
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Carboxyl Coupling Kit, 10 mL
bioWORLDbio-World's Carboxy Coupling Kit consists of amino-terminated Separopore® 4B-CL (4% crosslinked agarose beads). This kit is suitable for covalent immobilization of peptides or other carboxyl-containing (–COOH) molecules for use in affinity purification procedures. It includes carboxyl Coupling…
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High quality microplates coated with a high binding heparin solution. Heparin, Sodium salt is a low molecular weight heparin (LMWH) polymer that produces its major anticoagulant effect by activating antithrombin. This activation causes a conformational change in ATIII and allows for increased…
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Goat anti-Human IgG Nano-activeGold™
bioWORLDNano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™ reagents consist of affinity-purified IgG antibodies, goat anti-human IgG covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron…
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Biotin Coated Plates can be used as the basis for a variety of biomolecular assay techniques requiring the immobilization of probes that are conjugated with NeutrAvidin Protein, streptavidin, avidin or other biotin-binding proteins. Specifications: Biotin group accessible for binding…
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BSA (Bovine serum albumin)
bioWORLD1mg/ml BSA solution in 0.85% Sodium Chloride and 0.01% Sodium azide.
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bioWorld's Carboxylated 96-well Microplates contains a COOH-coated surface to which the amino group present in any molecules, such as peptides or proteins, binds to through formation of amide bonds by the action of carbodiimide. Applications: Coupling molecules, having an amino group, to…
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MagneZoom™ CAM (Calmodulin)
bioWORLDCalmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …
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Protease Inhibitor Cocktail
bioWORLDAEBSF E-64 Aprotinin EDTA Leupeptine Store -20°C Lyophillzed blend of five protease inhibitors for applications that require animal free reagents. Reconstitute with 1 ml water to produce a 100X concentrated solution. * Contains: AEBSF Aprotinin (recombinant) E-64 EDTA Leupeptin …
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Jacalin, belonging to the lectins family, is a Hemagglutinin from the seeds of the jack fruit Artrocarpus integrifolia . It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures. Jacalin shows specific…
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To remove non-specific binding of proteins to magnetic bead surface and to achieve clean co-immunoprecipitation of your desired antibody or any other ligand binding. Use it with any magnetic beads experiment. Size: 2-5µm. Silica-coated magnetic beads v pH stability: 4-9 Supplied as…
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Cu-IMAC Columns
bioWORLDCu-immobilized metal affinity chromatography (IMAC) columns consist of Cu-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Cu-IMAC Columns separate proteins via their differences in affinity to Cu2+ metal ions. Ideal for purification of recombinant…
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Protein-A Separopore® 4B
bioWORLDAffinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…
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Protein quantification is often required before precessing protein samples for analysis.The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+to Cu1+by proteins in an alkaline…
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ConA-MagneZoom Kit is designed for quick and efficient small scale purification by magnetic separation of macromolecules containing a-D-mannosyl or a-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides. The kit includes paramagnetic beads of average 5 µm in diameter covalently…
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BBS/Glycerol/Fish Gelatin
bioWORLDBuffered fish gelatin 0.05% blocks, diluents and coating solutions are supplied as 10X concentrates. Sold as 2 x 100mL or 500mL. pH range: 8.0 (+/- 0.1) formulated in borate buffered physiologic saline buffer containing glycerol as a preservative
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BlackPearl®-L (Wheat germ Lectin)
bioWORLDWheat germ lectin (Isolated from Triticum vulgaris) conjugated to BlackPearl® Magnetic Beads. Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor…
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Poly-L-Lysine-Coated Microplate
bioWORLDPoly-Lysine coated microplates High quality coated microplates. The Poly-Lysine coating of these 96-well microplates offers specific, high affinity binding to a variety of cell types.
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Epoxy Nanospheres MagneZoom™, 5 mL
bioWORLDSupplied as a 2.5% suspension MagneZoom™-Epoxy are silica-coated super paramagnetic beads with an Epoxy surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads. Magnetic bead ligand: Epoxy. Particle size:…
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MagneZoom™-PNA (Peanut Agglutini)
bioWORLDMagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…
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Protein-A Gold™
bioWORLDProtein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus . It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules,…
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Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…
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Nickel-Coated Microplates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing…
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Q-Cons™Rapid Protein Concentrators
bioWORLDQuick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume
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Cellbuster-A™ and Cellbuster-B™ Kits
bioWORLDCellBuster-A Kit (Animal cell protein prep.) An efficient cell lysis medium for animal cells for preparation of total cellular proteins. Cell lysis facilitated by combination of proprietary detergent mixture. The kit contains enough reagents for 50 medium scale cultures. …
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Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations. It is prepared by a modified method of the Cohn cold…