bioWORLD, NEW! Protein Expression
-
Goat anti-Human IgG Nano-activeGold™
bioWORLDNano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™ reagents consist of affinity-purified IgG antibodies, goat anti-human IgG covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron…
-
Bradford Reagent, Ready to Use
bioWORLDBradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
-
Amylose Separopore® (Agarose)
bioWORLDAn affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times. Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…
-
Biosilica™ beads
bioWORLDbioSilica Beads Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, a density of 1.96g/cm 3 , a refractive index (589nm) of about 1.37. Silica beads have high temperature stability. Also, because hydrophilic silica does not absorb proteins,…
-
Carboxy-terminated Magnetic Beads
bioWORLDSilica-coated uniform super paramagnetic beads with surface carboxyl group. Proteins and nucleic acids can be covalently bound to these beads with the retention of biological activity. Size: 5-10µm. COOH terminated Binding: up to 20mg/ml. pH stability: 4-9 Supplied as 25mg/ml…
-
Bovine Serum Albumin (BSA)
bioWORLDReagent grade, fatty acid free Moisture: < 1.0% Heavy metals: <0.5% Endotoxin: ≤0.1EU/mg pH (10% water): 6.5 - 7.5
-
Q-Cons™Rapid Protein Concentrators
bioWORLDQuick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume
-
Epoxy Nanospheres MagneZoom™, 5 mL
bioWORLDSupplied as a 2.5% suspension MagneZoom™-Epoxy are silica-coated super paramagnetic beads with an Epoxy surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads. Magnetic bead ligand: Epoxy. Particle size:…
-
To remove non-specific binding of proteins to magnetic bead surface and to achieve clean co-immunoprecipitation of your desired antibody or any other ligand binding. Use it with any magnetic beads experiment. Size: 2-5µm. Silica-coated magnetic beads v pH stability: 4-9 Supplied as…
-
Poly-L-Lysine-Coated Microplate
bioWORLDPoly-Lysine coated microplates High quality coated microplates. The Poly-Lysine coating of these 96-well microplates offers specific, high affinity binding to a variety of cell types.
-
Bioalginate beads
bioWORLDBioalginate Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent cations,…
-
Anti-biotin Goat IgG Nano-activeGold
bioWORLDNano-activeGold™ can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. Smallest commercially available gold immunoprobes. Penetrates and reaches antigens inaccessible to other probes. Extremely uniform 1.5 nm diameter gold label. …
-
COOH Nanospheres MagneZoom™, 5 mL
bioWORLDSupplied as a 2.5% suspension MagneZoom™-COOH are silica-coated super paramagnetic beads with an COOH surface group. bioWORLD’s MagneTons are suitable for high throughput screening Matrix: Silica coated magnetic beads. Magnetic bead ligand: COOH. Particle size: 1-2µm. …
-
The bioLifeTM MUG ß-Galactosidase Assay Kit provides a highly sensitive fluorogenic subsatrate for detection of ß-galactosidase activity in tranfected cells. In the presence of ß-galactosidase the colorless 4-methylumbelliferyl B-D-galactopyranoside (MUG) substrate is hydrolyzed…
-
Ni-alginate macro and micro beads
bioWORLDBioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…
-
Isopropyl-ß-D-Thiogalactopyranoside. An inducer of ß-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. Biotechnology…
-
BlackPearl®-L (Wheat germ Lectin)
bioWORLDWheat germ lectin (Isolated from Triticum vulgaris) conjugated to BlackPearl® Magnetic Beads. Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor…
-
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations. It is prepared by a modified method of the Cohn cold…
-
Zerobind™ Affinity Columns
bioWORLDZerobind™ affinity columns are designed to remove non-specific backgrounds from various polyclonal antibodies generally used for western blotting. Passing the diluted Antibody solution through Zerobind™ column can reduce non-specific binding while retaining activity against your…
-
Streptavidin Separopore® (Agarose)
bioWORLDApplications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…
-
Jacalin, belonging to the lectins family, is a Hemagglutinin from the seeds of the jack fruit Artrocarpus integrifolia . It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures. Jacalin shows specific…
-
Protease Inhibitor Cocktail
bioWORLDAEBSF E-64 Aprotinin EDTA Leupeptine Store -20°C Lyophillzed blend of five protease inhibitors for applications that require animal free reagents. Reconstitute with 1 ml water to produce a 100X concentrated solution. * Contains: AEBSF Aprotinin (recombinant) E-64 EDTA Leupeptin …
-
Applications: OnePass™ GST-Tagged Recombinant Protein Purification column consists of Glutathione-Separopore® 4B-CL conveniently prepacked column cartridges for low pressure flow chromatography. This product provides a one step purification method and permits rapid, mild and highly selective…
-
Wheat germ -Triticum vulgaris- agglutinin (WGA) linked to MagneZoom (magnetic beads). For magnetic separation by binding specifically to cell particles, glycoconjugates and polysachharides containing N-acetylglucosamine residues. Size: 2-5µm. Ligand: Wheat germ agglutinin Ligand…
-
bioPLUS D (+)-Trehalose dihydrate
bioWORLDAn osmolyte, chemical chaperone, and inducer of autophagy. Synonym: α-D-Glucopyranosyl-α-D-glucopyranoside, α,α-Trehalose Molecular Weight: 378.33 g/mol Molecular Formula: C12H22O11•2H2O CAS: 6138-23-4 …
-
Streptavidin Coated Plates are available in 96-well plate format, and pre-blocked with BSA Blocking Buffer. bio-world Biotin-Binding Plates are available in clear to be used with colorimetricdetection methods. Easy and gentle immobilization of biotin-containing conjugates…
-
Protein quantification is often required before precessing protein samples for analysis.The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+to Cu1+by proteins in an alkaline…
-
-
Cu-alginate macro and micro beads
bioWORLDBioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…
-
Nickel-Coated Microplates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing…
-
MagneZoom™ CAM (Calmodulin)
bioWORLDCalmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …
-
Protein-A Separopore® 4B
bioWORLDAffinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…