bioWORLD, NEW! Protein Expression

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  • bioWold's Streptalon (Streptavidin magnetic beads) Kit is designed for convenient and quick purification of biotinylated proteins and nucleic acids in immunoprecipitation, protein interaction studies, immunoassays and cell isolation, etc. Streptalon (Streptavidin Magnetic Beads) Kit…

  • Protein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus . It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules,…

  • bio-World's Carboxy Coupling Kit consists of amino-terminated Separopore® 4B-CL (4% crosslinked agarose beads). This kit is suitable for covalent immobilization of peptides or other carboxyl-containing (–COOH) molecules for use in affinity purification procedures. It includes carboxyl Coupling…

  • Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…

  • Erythrina cristagalli (coral tree) lectin is a 54,000 dalton glycoprotein consisting of two different subunits of approximately 28,000 and 26,000 daltons. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl ( b -1,4)…

  • Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…

  • Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • bioSilica Beads Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, a density of 1.96g/cm 3 , a refractive index (589nm) of about 1.37. Silica beads have high temperature stability. Also, because hydrophilic silica does not absorb proteins,…

  • bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

  • Quick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume

  • Streptavidin Coated Plates are available in 96-well plate format, and pre-blocked with BSA   Blocking Buffer. bio-world Biotin-Binding Plates are available in clear to be used with colorimetricdetection methods. Easy and gentle immobilization of biotin-containing conjugates…

  • Biotin Coated Plates can be used as the basis for a variety of biomolecular assay techniques requiring the immobilization of probes that are conjugated with NeutrAvidin Protein, streptavidin, avidin or other biotin-binding proteins. Specifications: Biotin group accessible for binding…

  • Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…

  • Source: E.Coli Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ~3…

  • The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an…

  • From Streptomyces avidini Biotechnology Grade Activity: >17U/mg protein Electrophoresis: Pass DNase and RNase Activity: None detected Protease: None detected Product for in vitro and lab research use only, not for human use or consumption

  • An affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP).  Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times.  Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…

  • Bioalginate Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent cations,…

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    Cu-immobilized metal affinity chromatography (IMAC) columns consist of Cu-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Cu-IMAC Columns separate proteins via their differences in affinity to Cu2+ metal ions. Ideal for purification of recombinant…

  • Wheat germ -Triticum vulgaris- agglutinin (WGA)  linked to MagneZoom (magnetic beads). For magnetic separation by binding specifically to cell particles, glycoconjugates and polysachharides containing N-acetylglucosamine residues. Size: 2-5µm. Ligand: Wheat germ agglutinin  Ligand…

  • Calmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …

  • Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…

  • Recombinant protein G covalently linked to MagneZoom (paramagnetic beads). Protein G binds Rc region of mammalian immunoglobulin. Ideal for purification and fractionation of IgG subclasses that poorly bind protein A. Suitable for high throughput screening. Particle size: 2-5µm Ligand:…

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    A biotin-binding glycoprotein prepared from chicken egg white, Molecular weight=67kDa, pI = 10 Glycosylation and isoelectric point make avidin more soluble than streptavidin Significantly less expensive than recombinant streptavidin protein Based on strong binding with biotin, avidin is…

  • AEBSF E-64 Aprotinin EDTA Leupeptine Store -20°C Lyophillzed blend of five protease inhibitors for applications that require animal free reagents. Reconstitute with 1 ml water to produce a 100X concentrated solution. * Contains: AEBSF Aprotinin (recombinant) E-64 EDTA Leupeptin …

  • 1mg/ml BSA solution in 0.85% Sodium Chloride and 0.01% Sodium azide.

  • MagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…

  • Supplied as a 2.5% suspension MagneZoom™-Epoxy are silica-coated super paramagnetic beads with an Epoxy surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads. Magnetic bead ligand: Epoxy. Particle size:…

  • Buffered fish gelatin 0.05% blocks, diluents and coating solutions are supplied as 10X concentrates. Sold as 2 x 100mL or 500mL. pH range: 8.0 (+/- 0.1) formulated in borate buffered physiologic saline buffer containing glycerol as a preservative

  • Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations. It is prepared by a modified method of the Cohn cold…

  • Mixture of six protease inhibitors for use with mammalian cells and tissue extracts. Supplied in l ml DMSO. Will treat proteases extracted from 20g tissue. Contains: AEBSF, E-64, Aprotinin, Leupeptin, Bestatin, Pepstatin A Specificity: Serine Proteases, Cysteine Proteases, Trypsin like…

  • Nano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™  reagent consist of Streptavidin covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. …

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