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GST-Tagged Recombinant Protein Purification OnePass™ Column 1 x 1 ml, 1 mL

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OnePass™ GST-Tagged Recombinant Protein Purification column consists of Glutathione-Separopore® 4B-CL conveniently prepacked column cartridges for low pressure flow chromatography. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated highly crosslinked 4% agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity and could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel. Reduced glutathione is covalently linked Separopore® 4B-CL for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function. The OnePass™ prepacked Glutathione- Separopore® spin columns simply purification of GST-tagged proteins.

Ligand: Reduced glutathione (GSH)
Matrix: Separopore® 64B-CL (highly crosslinked agarose beads, 4%)
Average Particle size: 52 - 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix activation: Epoxy
Matrix spacer: 12 atoms
Ligand density: 5 – 10 mg GST / ml drained gel
Binding capacity: 8 mg recombinant GST-tagged protein / ml drained gel
pH stability: 3 – 12
Storage: 2 °C – 8 °C 

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GST-Tagged Recombinant Protein Purification OnePass™ Column 1 x 1 ml, 1 mL
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