bioWORLD, 500 g, Standard Microbiological Media
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Tryptic Soy Agar
bioWORLDUsed for the isolation and cultivation of nonfastidious and fastidious microorganisms Can be prepared with or without blood or enrichments pH: 7.3 Working Concentration: 40 g/L Dehydrated Appearance: light beige, homogeneous powder Prepared Appearance: Plain – light…
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For the isolation and cultivation of lactic streptococci Does not contain β-glycerophosphate or lactose pH: 7.0 Dehydrated Appearance: beige to medium tan, homogeneous powder Prepared Appearance: medium amber broth Contains (per liter): Tryptone: 2.5g …
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Standard Methods Agar, 500 g
bioWORLDUsed for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance Made according to the American Public Health Association (APHA) formulation pH: 7.0 Working Concentration: 23.5 g/L Dehydrated Appearance: light beige,…
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Beef Extract (Powder), 500 g
bioWORLDRecommended for use as a nutrient at 0.3-0.5% concentration in various culture media Can be used in fermentation studies as it is free of fermentable substrate pH: 7.0 Dehydrated Appearance: cream to tan, homogeneous powder Prepared Appearance: yellow to tan Soluble in water
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Wilkins Chalgren Agar, 500 g
bioWORLDUsed to determin minimal inhibitory concentration (MIC’s) of antibiotics for anaerobic bacteria by the agar dilution procedure pH: 7.1 Working Concentration: 48 g/L pDehydrated Appearance: light beige, homogeneous powder Prepared Appearance: yellow-beige agar Soluble…
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R2B Broth
bioWORLDLow nutrient medium for plate detection of heterotropic bacteria in water Low concentrations of carbon and energy sources enhance the recovery of stressed bacteria Ideal for membrane filtration methods pH: 7.2 Working Concentration: 3.6 g/L Dehydrated Appearance: light…
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Used in the selective isolation and cultivation of Clostridium difficile To be prepared with the addition of blood, cycloserine, and cefoxitin pH: 7.4 Dehydrated Appearance: light beige, homogeneous powder Prepared Appearance: light to medium amber broth Soluble in…
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Pancreatic Digest of Casein, 500 g
bioWORLDGeneral purpose growth peptone used for the cultivation of most bacteria and fungi and some protozoa Can be used for indole testing because of its high tryptophan content Is biologically free of carbohydrates and is recommended for fermentation studies Can be used to determine…
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A-1 Medium, 500 g
bioWORLDUsed in the detection of coliform microorganisms in water and food products. pH: 6.9 Dehydrated Appearance: light beige, lumpy powder Prepared Appearance: Light amber Soluble in boiling water Composition (g/L): Sodium Chloride:5g Lactose: 5g Salicin: 0.1g …
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Luria-Bertani (LB) Agar, Miller
bioWORLDFor propagation and maintenance of Escherichia coli Contains twenty times the sodium chloride level of Luria Agar Base, Miller and twice the level found in LB Agar, Lennox pH: 7.0 Working Concentration: 40 g/L Dehydrated Appearance: light tan, homogeneous powder Prepared Appearance: light…
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Brain-Heart Infusion Agar
bioWORLDUsed in the cultivation of a wide variety of microorganisms including bacteria, yeasts and molds With the addition of 5% or 10% sheep blood, it is used for the isolation and cultivation of a wide variety of fungal species from clinical and nonclinical sources pH: 7.4 Dehydrated…
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Used for cultivating microorganisms from highly viscous or gelatinous materials pH: 7.2 Working Concentration: 25 g/L Dissolve in 960 mL purified water with 40 mL polysorbate 20 Dehydrated Appearance: beige, homogeneous powder Prepared Appearance: light amber broth …
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Super Optimal Broth (SOB Medium)
bioWORLDUsed for cultivation of recombinant strains of E. Coli Is a nutritionally rich growth medium for use in the preparation and transformation of competent cells pH: 7.0 Working Concentration: 28 g/L Dehydrated Appearance: light beige, homogenous powder Prepared Appearance:…
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Oxford Listeria Agar, 500 g
bioWORLDUsed with addition of antibiotics for the selective isolation of Listeria monocytogenes from food pH: 7.2 Working Concentration: 55.5 g/L Dehydrated Appearance: beige, homogeneous powder Prepared Appearance: light to medium amber agar Soluble in boiling water …
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Mannitol Salt Agar, 500 g
bioWORLDSelective agar medium used for the identification and isolation of pathogenic staphylococci pH: 7.4 Working Concentration: 111 g/L Dehydrated Appearance: light pink, homogeneous powder Prepared Appearance: pinkish red agar Soluble in boiling water Composition (g/L): …
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Simmons Citrate Agar, 500 g
bioWORLDUsed as a differential medium for the identification of enteric bacteria, which utilize citrate as the sole source of carbon ph: 6.9 Working Concentration: 24.2 g/L Dehydrated Appearance: green, homogeneous powder Prepared Appearance: medium to dark green agar Soluble in…
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Columbia Blood Agar Base
bioWORLDUsed for the cultivation and determining of hemolytic reactions of fastidious and non-fastidious microorganisms Supplemented with 5-10% sheep, rabbit, or horse blood pH: 7.3 Dehydrated Appearance: beige, homogeneous powder Prepared Appearance: without blood- light to medium…
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Mueller Hinton Agar
bioWORLDFor antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardized by the National Committee for Clinical Laboratory Standards (NCCLS) pH: 7.3 Working Concentration: 38 g/L Dehydrated Appearance: beige, homogeneous…
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Agar, Bacteriological
bioWORLDFine, light-colored granules Bacteriological Grade Gel Strength: 550–950 g/cm2 Gelling Temperature: 34–38 °C Melting Temperature: 84–88 °C pH: 6.0–7.5 Soluble in boiling water
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Yeast Nitrogen Base
bioWORLDFor classifying yeasts based on carbon assimilation Requires addition of a carbon source pH: 5.4 Working Concentration: 6.7 g/L (single-strength) or 67 g/L (10X) Dehydrated Appearance: off-white, homogeneous powder Prepared Appearance: single-strength solution is colorless…
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Brucella Agar
bioWORLDRecommended for the cultivation of Brucella species and other fastidious microoorganisms Can be used to determine bacterial hemolytic reactions with the addition of blood pH: 7.0 Dehydrated Appearance: light beige, homogeneous powder Prepared Appearance: yellow beige agar …
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Bacto Peptone (Powder)
bioWORLDUsed as an organic nitrogen source in microbiological media for culturing variety of bacteria and fungi Enzymatic digest of animal protein Working concentration: 50 mg/mL Dehydrated Appearance: faint yellow to yellow-brown powder Prepared Appearance: dark yellow solution …
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Yeast Extract
bioWORLDAqueous extract of autolysed brewer's yeast Has a high vitamin content, providing excellent growth factors for most microorganisms Is usually added to culture media to provide a source of vitamins at a concentration of 3-5 g/L After opening, tightly recap and place the container…
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BCYE Agar (Legionella Agar), 500 g
bioWORLDCommonly used in primary isolation of Legionella from environmental and clinical materials pH: 6.85 Dehydrated Appearance: dark grey to black, homogeneous powder Prepared Appearance: black-green to gray-black agar Soluble in boiling water Contains: (g/L: Yeast…
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Nutrient Broth
bioWORLDRecommended for the cultivation of non-fastidious microorganisms according to the methods described by the American Public Health Association pH: 6.8 Working Concentration: 8 g/L Dehydrated Appearance: medium tan, homogeneous powder Prepared Appearance: light to medium amber…
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Cystine Heart Agar
bioWORLDUsed with hemoglobin for cultivating Francisella tularensis and without enrichment for cultivating gram-negative cocci and other microorganisms. pH: 6.8 Dehydrated Appearance: beige, homogeneous powder Prepared Appearance: Plain – light to medium amber agar W/ Hemoglobin…
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Malt Extract Broth
bioWORLDMalt Extract Broth Used for the cultivation and maintenance of yeasts and molds pH: 4.7 Working Concentration: 15 g/L Dehydrated Appearance: light beige to beige, homogeneous powder Prepared Appearance: light amber broth Soluble in water Contains: Malt Extract Base Maltose Dextrose Yeast Extract…
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Czapek Dox Agar
bioWORLDUsed for fungi and bacteria that are unable to utilize sodium nitrate a their sole source of nitrogen Working Concentration: 50.1 g/L pH: 7.3+/-0.2 Dehydrated Appearance: off-white to beige powder Prepared Appearance: colorless agar Soluble in boiling water Contains: …
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Used with antimicrobics for the selective enrichment of Campylobacter species Supplement with Cefoperazone, Cycloheximide, Trimethoprim, and Vancomycin to increase selectivity(not included in formulation) May be enriched with Lysed Horse Blood (not included in formulation) pH: 7.4 …
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Lactobacilli MRS
bioWORLDFor the cultivation of Lactobacillus species from oral, fecal and other specimen sources pH: 6.5 Working Concentration: 69 g/L. Add 1ml of Tween 80 per liter of media. Dehydrated Appearance: light tan, homogeneous powder Prepared Appearance: amber agar Soluble in boiling…
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Lauryl Sulfate Broth, 500 g
bioWORLDFor the detection of coliforms in water, wastewater and foods pH: 6.8 Working Concentration: 35.6 g/L Dehydrated Appearance: light beige, homogeneous powder Prepared Appearance: tan to yellow broth Soluble in water Composition: Pancreatic Digest of Casein …
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Eosin Methylene Blue Agar (H-H&T)
bioWORLDSlightly selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric bacilli pH: 7.2 Working Concentration: 36 g/L Dehydrated Appearance: light red-purple, homogeneous powder Prepared Appearance: dark blue purple agar …