FCCP [Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] is a potent uncoupler of oxidative phosphorylation in mitochondria. It is used as a positive control for mitochondrial membrane depolarization. FCCP disrupts the synthesis of ATP by trnasporting protons across mitochondrial inner…

Coelenterazine, native; 2-(p-Hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzyl-imidazo[1,2-a]pyrazin-3-(7H)-one MOLECULAR BIOLOGY GRADE Coelenterazine is a luminophore found in aquatic organisms and is the substrate for many luciferase enzymes, including Renilla reniformis, Gaussia, and…

Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal…

Tested and Certified for in vivo imaging  Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of the luciferase. This substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors and emits a characteristic yellow-green emission in the…

N-alpha-L-rhamnopyranosyloxy(hydroxyphosphinyl)-L-Leucyl-L-Tryptophan Specificity: Inhibits some metalloproteases, including thermolysin, collagenase and bacterial metallo proteases from Bacillus subtilis, Streptomyces griseus and Pseudomonas aeruginosa (metallo elastase). Solubility: …

Bovine serum albumin is a single polypeptide of 67kDa and consists of 59 lysine residues, of which 30-35 have primary amines that can react with crosslinkers for the coupling of peptides. OneQuant ™ BSA The BSA carrier protein is also available as our single use OneQuant ™ vials…

Calpain inhibitor I; N-[N-(N-Acetyl-L-leucyl)-L-leucyl]-L-norleucine Specificity: Cell permeable peptide aldehyde inhibitor of calpain I and to a lesser extent calpain II. Also inhibits other neutral cysteine proteases, cathepsin B and L and the proteasome. Solubility: Soluble in DMSO or…

Keyhole limpet hemocyanin is a widely used carrier protein due to its large molecular mass (4.5x10 5 -1.3x10 7 Da aggregates) and large number of primary amines that can react with cross-linkers for the coupling of peptides. The KLH is supplied as a clear to slightly hazy, bluish solution in…

N-[(2S,3R)-3-Amino-2-hydroxy-4-phenylbutyryl]-L-leucine hydrochloride Specificity: Competitive inhibitor of surface aminopeptidases, including aminopeptidase B (K_i=2nM), leucine aminopeptidase (K_i=20nM). Also inhibits aminopeptidases N; does not inhibit endoproteases. …