Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make…

Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of…

This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach the basic principles of a gel box, specifically horizontal agarose gel electrophoresis. The kit is supplied with all the reagents necessary to conduct 4 gel box science experiments, each using a gel box (not supplied). …

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate and distinguish proteins by…

G-Sep™ Phenyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with phenyl groups for Hydrophobic Interaction Chromatography (HIC). The resin is used to separate biomolecules on the basis of relative hydrophobicity. G-Sep™ Phenyl Agarose Fast Flow is available in two levels of…

The 5ml empty, plastic, disposable columns have an internal volume of 6.5ml and is designed for small scale gravity flow purifications. The resin bed volume is 2.5ml. Supplied with caps and stoppers. Features Total Volume: 6.5ml Resin Volume: 2.5ml Reservoir Volume: 4ml …

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples. …

A selection of genomic isolation kits are offered that purify high quality genomic DNAfrom a wide variety of sources and for a wide array of applications. Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications. These kits are fully…

G-Sep™ SP Agarose Fast Flow (FF) resin is a strong cation exchanger composed of highly cross-linked 6% agarose beads, with Sulphopropyl (SP) strong cation exchange groups. The resin is extensively used for biomacromolecule separations in both research and industrial applications. The…

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Cobalt Chelating Resin is specifically designed for the…

An interesting hands-on lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handling techniques and cultivation of bacteria.   Using bacterial culture techniques, students discover and isolate the bacteria…

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer…

Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Features Single step extraction protocol. …

The XIT ™ Genomic DNA fromBuccal Cellskit is designed for the isolation of genomic DNA from Buccal cells (cheek cells). The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestionand complete protein precipitation, followed by DNA…

G-Trap™ FPLC Columns are empty columns designed for fast protein liquid chromatography. G-Trap™ FPLC Columns are made of polypropylene and come in 1 ml and 5 ml sizes. Features: Column material:  polypropylene   (PP) Frit material:  Ultra high…

G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strongcation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)…

A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a…

A selection of genomic isolation kits are offered that purify high quality genomic DNA from a wide variety of sources and for a wide array of applications. Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications. These kits are…

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation.  Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyzes the formation of phosphodiester bonds. …

Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. The Columns are available…

The XIT ™ Genomic DNA fromBlood kit is designed for the isolation of genomic DNA from blood, bone marrow and buffy coat. The kit usesfour main steps (see figure) that are red blood cell lysis, rapid & efficient nuclear lysis, complete protein precipitation, followed by DNA…

The Fab & F(ab)₂ Fragmentation of Mouse IgG₁ kit is designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilizes our Immobilized Ficin resin. Ficin (or Ficain) (~25,000Da) is a cysteine…

Today’s media routinely covers forensic stories of criminals captured and innocents released using the powerful DNA fingerprinting technique. This kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a…

G-Trap™ Octyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC). Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of…

HOOK™-PEG₂-Iodoacetyl-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups.…

Nucleic acids (DNA and RNA) and proteins are routinely resolved by electrophoresis, which separates molecules based on size and/or charge. Following electrophoresis, the molecules need to be extracted from the electrophoresis medium for use in downstream applications and experiments.…

An important and essential tool in protein analysis is electrophoresis.  Protein electrophoresis allows researchers to separate complex protein samples by their molecular size and/or net charge on polyacrylamide gels.  The proteins can be subsequently visualized with various protein…

G-Biosciences C18 Spin Columns are ready-to-use micro centrifuge columns for peptide clean up and concentration.The columns consist of porous C18 reverse-phase resin that has a particle size if ~15µm and a pore size of 300Å.The resin offers highly efficient binding and recovery of…

These empty, plastic, disposable 1ml spin columns have an internal volume of ~0.7ml and is designed for small scale purifications using 1.5 and 2ml centrifuge tubes. Supplied with cap Features Resin Volume: 700µl Total Volume: 1.1ml Reservoir Volume: 400µl …

Dialysis is a routinely used technique in research laboratories to “change” the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as…

EndotoxinOUT™ was designed for the rapid removal of endotoxins and pyrogens (LPS, lipopolysaccharides) from nucleic acid (DNA) samples. EndotoxinOUT™ is an ideal product for the cleanup of buffers, cell culture media, protein solutions, nucleic acid (DNA) samples and pharmacological…