A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilize difficult proteins.
The FOCUS™ Soluble & Insoluble kit is supplied with a specific clean-up kit for the preparation of each fraction for isoelectric focusing and 2D electrophoresis for improved spot resolution.FOCUS™ Soluble & Insoluble kit is designed for 50 preps, where 1 prep is:
- 100mg mammalian tissue
- 50µl wet animal cell pellet
- 50µl wet yeast pellet
- 50µl wet bacteria pellet
- 250mg plant tissue
Features
- Generates soluble and insoluble fractions.
- Fractions fully compatible with 2D electrophoresis.
Applications
- Extraction of soluble and insoluble proteins from tissues, cells, plants, yeast, bacteria and other sources.
- Suitable for sample preparation for 2D gel electrophoresis and other applications.