Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyzes the formation of phosphodiester bonds.
The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.
The kit is also designed to be used with the DNA fragments produced with the “DNA Restriction Digestion” kit and purified with the “Purification of a Gene”. This will result in the generation of a viable plasmid that can be transformed into bacteria with the “Bacterial Transformation” kit.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
Applications
- Teaches joining of DNA ends by a process known as ligation.
- Contains all the reagents necessary for ligation.