Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse…

Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of…

Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…

Blasticidin S HCl is produced by Streptomyces griseochromogenes and inhibits translation in both prokaryotes and eukaryotes, thus preventing the formation of peptide bonds. Appearance: White powder Melting Point: 218.0–225.0°C (424.4-437.0°F) Water Solubility: Soluble

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging.  Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action.  EDTA sequesters these ions and thus enhances the efficacy of…

Sodium bicarbonate Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment.  Bicarbonate is a weak buffer with that makes maintaining a pH range of 7.2 - 7.6 somewhat difficult, but it is non-toxic, and is a…

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…

Thomas No. B003K43 A modified forumulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites. Complete Certificate of Analysis available…

Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…

Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with…

Reagent grade water is ideal for the preparation of reagents, rinsing glassware and plastic ware, cleaning electrodes and other common laboratory tasks requiring highly pure water. No toxic agents, including DEPC, are used in its preparation. The design allows for portability, ease of storage and…

MCDB 131 was originally developed by the researchers Knedler and Ham for the low-protein and serum free growth of human microvascular endothelial cells (HMVEC).  Since its development, this medium has been used for this cell type and others such as liver, smooth muscle, cardiac myocytes. …

Ham's F-10 is a classical media designed to support the growth of a variety of cell types, including HeLa, Chinese Hamster Ovary (CHO), primary cells from tissue explants and various types of human diploid cells. 

Lymphocyte Separation Medium (LSM) was originally designed for the in vitro isolation of lymphocytes from diluted whole blood.  It is a sterile, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/mL at 20ºC. Complete Certificate of…

L-glutamin L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds.…

Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…

Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…

Corning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications. Laminin is a major component of basement membranes. It has numerous biological activities ­including promotion of cell adhesion, migration, chemotaxis, growth, and…

For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone.  Corning BioCoat PDL/LM and PLO/LM Cultureware is…

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…

Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…

Corning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…

This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. The product is a…

Corning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications.

The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and…

Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…

Interleukin-2 (IL-2), mouse recombinant, plays a central role in cell-mediated immune response. Was first identified by its ability to promote the long-term proliferation of activated T-cells. (25,000 BRMP units) References: 1. Hatakeyama, M. and Taniguchi, T., "Interleukin-2" in Peptide Growth…

The Corning® Mediatech™ cell culture media is formulated with high industrial standards for excellent results. It maintains cell tonicity and viability. Application: For Research Use Only

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…

Laminin (LM), a major component of basement membranes, is a multifunctional glycoprotein that is used as a substrate to culture and maintain differentiated function of a wide variety of cells. Laminin has been shown in culture to stimulate neurite outgrowth, promote cell attachment, chemotaxis,…

Ham's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids.  This medium is also suitable for carcinoma cells, rat skeletal myoblasts,…