Substrates, 1 g, Chemicals
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o-Nitrophenyl-ß-D-Galactopyranoside
MP Biomedicalso-Nitrophenyl-β-D-galactoside (ONPG) is a colorimetric substrate for b-galactosidase. This product is hydrolyzed to o-nitrophenol by the enzyme. o-Nitrophenol is yellow in alkaline solution and can be quantitated at 410-420 nm 2 . o-Nitrophenyl-β-D-galactoside is a cell culture…
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Isopropyl-ß-D-Thiogalactopyranoside. An inducer of ß-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. Biotechnology…
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D-Luciferin Firefly, potassium salt
G-BiosciencesTested and Certified for in vivo imaging Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors to emit a characteristic yellow-green emission in…
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4-Methylumbelliferyl caprylate
G-Biosciences4-Methylumbelliferyl caprylate Octanoic acid 4-methylumbelliferyl ester 4-MU-caprylate MOLECULAR BIOLOGY GRADE White to off white crystalline powder Fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Used for the…
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4-Nitrophenyl-alpha-L-fucopyranoside
G-Biosciences4-Nitrophenyl-α-L- fucopyranoside p-Nitrophenyl-α-L-fucoside PNP-α-L-Fuc MOLECULAR BIOLOGY GRADE White to off white crystalline powder Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of…
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Iodonitrotetrazolium Chloride (INT)
G-BiosciencesIodonitrotetrazolium chloride (INT) is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. INT dye is often used to visualize dehydrogenase activity histochemically as well as western blot staining and…
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(X-glu, X-glcA, BCIG) 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt MOLECULAR BIOLOGY GRADE Purity: greater than 99% (HPLC) Thin Layer Chromatogram: single spot Biological Activity: indigo-blue assay Elemental…
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L-Lysine 7-amido-4-methylcoumarin, acetate salt
G-BiosciencesL-Lysine 7-amido-4- methylcoumarin acetate salt H-Lys-AMC · AcOH MOLECULAR BIOLOGY GRADE Off white to white crystalline powder Forms a colorless, clear solution when dissolved 5% in water. Substrate for aminopeptidase b yielding a blue…
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Glutathione, Reduced
MP BiomedicalsGlutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is…
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5-Bromo-4-chloro-3-indoxyl-2-acetamido-2-deoxy-β-D-galactopyranoside; 5-Bromo-4-chloro-3-indoxyl-N-acetyl-β-D-galactosaminide X-β-D-GalNAc MOLECULAR BIOLOGY GRADE Used as a chromogenic substrate for N-Acetyl-β-D-galactosaminidase, yielding a blue precipitate. It is also…
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2-Naphthyl caprylate
G-Biosciences2-Naphthyl caprylate Octanoic acid β-naphthyl ester; β-Nap-caprylate MOLECULAR BIOLOGY GRADE White to off-white crystalline powder Chromogenic substrate for carboxylesterase and lipase. 2-naphthol is…
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5-Bromo-4-chloro-3-indoxyl sulfate, potassium salt
G-BiosciencesX-Sulfate potassium salt; 5-Bromo-4-chloro-3-indoxyl sulfate, potassium salt MOLECULAR BIOLOGY GRADE X-Sulfate K is a slightly off-white to faint-yellowish powder that is used as a chromogenic substrate for aryl-sulfatase. X-Sulfate K yields a blue precipitate when cleaved. Store at…
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2-Naphthyl-alpha-L-fucopyranoside
G-Biosciences2-Naphthyl-α- L-fucopyranoside β-Naphthyl-α-L-fucoside β-Nap-α-L-Fuc MOLECULAR BIOLOGY GRADE White to off-white crystalline powder Chromogenic substrate for α-L-Fucosidase. 2-naphthol is released upon…
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4-Nitrophenyl-beta-D-maltopyranoside
G-Biosciences4-Nitrophenyl-β-D- maltopyranoside p-Nitrophenyl-β-D-maltoside PNP-β-D-maltoside MOLECULAR BIOLOGY GRADE White to off white crystalline powder Chromogenic β-maltosidase substrate, yielding a yellow solution upon cleavage. Store at…
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Ketoprofen [2-(3-Benzyoylphenyl) propionic acid
G-BiosciencesKetoprofen is one of the propionic acid class of nonsteroidal anti-inflammatory drug (NSAID) with analgesic and antipyretic effects. Ketoprofen is used for its antipyretic, analgesic, and anti-inflammatory properties by inhibiting cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes reversibly,…
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4-Methylumbelliferyl palmitate
G-Biosciences4-Methylumbelliferyl palmitate Palmitic acid 4-methylumbelliferyl ester 4-MU-Pal MOLECULAR BIOLOGY GRADE White to off-white powder Fluorogenic substrate for esterase, yielding a blue fluorescent solution upon cleavage. Store at -15°C in the dark. …
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4-Aminophenyl-beta-D-galactopyranoside
G-Biosciences4-Aminophenyl-β-D-gal MOLECULAR BIOLOGY GRADE 4-Aminophenyl-β-D-galactopyranoside is a substrate for β-galactosidase. It is also a weak β-D-galactosidase inducer and can be used in affinity chromatography for isolation of galactose-binding. The 4-aminophenyl…
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L-Tryptophan 7-amido-4-methylcoumarin hydrochloride
G-BiosciencesL-Tryptophan 7-amido-4-methylcoumarin hydrochloride H-Trp-AMC · HCl MOLECULAR BIOLOGY GRADE White to off white powder Forms a clear, colorless solution when dissolved 0.5% in methanol/water (1:1) Substrates of glycosidases, phosphatases and esterases are commonly used in…
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4-Nitrophenyl-alpha-D-maltohexaoside
G-Biosciences4-Nitrophenyl-α-D- maltohexaoside p-Nitrophenyl-alpha-D-maltohexaoside PNP-alpha-D-maltohexaoside MOLECULAR BIOLOGY GRADE Off-white to yellow crystalline powder Chromogenic substrate for α-amylase, yielding a yellow solution upon cleavage. …
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2-Naphthyl-beta-D-galactopyranoside
G-Biosciences2-Naphthyl-β- D-galactopyranoside β-Naphthyl-β-galactoside; β-Nap-β-D-Gal MOLECULAR BIOLOGY GRADE White to off-white crystalline powder 2-Naphthyl-beta-D-galactopyranoside is a substrate for beta-galactosidase. 2-naphthol is released upon…
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N-Tosyl-L-alanine 3-indoxyl ester
G-BiosciencesTos-Ala-Y Handling Store at -15°C, protect from light. PubChem Chemical ID: 53471583 Features Synonyms: H-TOS-ALA-Y, N-TOSYL-L-ALANYLOXYINDOLE, 1H-indol-3-yl N-[(4-methylphenyl)sulphonyl]-L-alaninate, N-[(4-Methylphenyl)sulfonyl]-L-alanine 1H-indol-3-yl ester,…
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4-Methylumbelliferyl-N-acetyl-beta-D-galactosaminide
G-Biosciences4-Methylumbelliferyl-N- acetyl-β-D- galactoside 4-Methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside 4-MU-beta-D-GalNAc MOLECULAR BIOLOGY GRADE White to off white powder Fluorogenic substrate for N-acetyl-beta-D-galactosaminidase.…
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L-Glutamic acid alpha-(7-amido-4-methylcoumarin)
G-BiosciencesL-Glutamic acid α-(7-amido-4-methylcoumarin) H-Glu-AMC MOLECULAR BIOLOGY GRADE White to off white powder Forms a colorless, clear solution when dissolved 1% in 80% acetic acid. Fluorogenic substrate for aminopeptidase a yielding a blue fluorescent solution upon cleavage. Store…
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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate. X-Gal is a mainstay in molecular biology cloning applications in which it is…
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MUG
G-BiosciencesMug; 4-Methylumbelliferyl beta-D-glucuronide Hydrated form of MUG is CAS # 881005-91-0. MOLECULAR BIOLOGY GRADE 4-methylumbelliferyl-beta-D-glucuronide (MUG) is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase…
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4-Methylumbelliferyl-alpha-D-glucopyranoside
G-Biosciences4-Methylumbelliferyl-α-D-glucopyranoside 4-MU-α-D-Glc EINECS: 241-794-0 Beilstein: 1690776 S-Phrases: S24/25,S22 MDL: MFCD00067661 Features CAS#: 17833-43-1 Molecular Formula: …
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5-Bromo-4-chloro-3-indoxyl-alpha-D-mannopyranoside
G-BiosciencesY-Choline phosphate 3-Indoxyl choline phosphate Y™-phos-choline, Y™-CP MOLECULAR BIOLOGY GRADE Off-white crystalline powder Microbial activity indicator Chromogenic substrate for phosphatidylcholine-specific phospholipase C, yielding a blue precipitate upon cleavage. …
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Magenta-phos·2Na·H2O; 5-Bromo-6-chloro-3-indoxyl phosphate, disodium salt monohydrate MOLECULAR BIOLOGY GRADE Magenta phosphate disodium salt monohydrate is an off-white powder that is used to visualize phosphatase activity. The end product is insoluble and magenta colored when…
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2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl…
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4-Nitrophenyl-alpha-D-maltopyranoside
G-Biosciences4-Nitrophenyl-α-D- maltopyranoside p-Nitrophenyl-alpha-D-maltoside PNP-alpha-D-maltoside MOLECULAR BIOLOGY GRADE White to off white crystalline powder Chromogenic α-maltosidase substrate, yielding a yellow solution upon cleavage. Store at…
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H-Arg-Arg-AMC · 3 HCI MOLECULAR BIOLOGY GRADE Fluorogenic substrate for cathepsin B or aminopeptidase III. Yields a blue fluorescent solution upon cleavage. Store at -15°C and protect from light. Features CAS#: 201847-69-0 Molecular Formula: …
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L-Arginine 7-amido-4-methylcoumarin dihydrochloride
G-BiosciencesH-Arg-AMC·2HCl MOLECULAR BIOLOGY GRADE Excellent flourogenic substrate for quantitative determination of cathepsin H activity. Also a useful substrate for aminopeptidase B. It yields blue fluorescent solution upon cleavage. Store in -15°C. Protect from light. Features …