2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
2,2-Azino-bis-(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) has been used as a chromogenic substrate for horseradish peroxidase (HRP), both in general activity assays and in ELISA applications. Activity of HRP using ABTS appears about four-fold higher than using pyrogallol. It is mainly used as a substrate in sensitive peroxidase assays.
Suitability: Suitable for use as a reagent for lactoperoxidase
Key Applications: Chromogenic substrate for sensitive peroxidase assays.
Application Areas: Molecular Biology
Product Type: Biochemicals
Biochemical Category: Biochemicals
Extinction Coefficient (E1%): EmM(342 nM) > 37.4; EmM(225 nM) > 48.15; EmM(414 nM) = 36 (Lit.)
UV/Visible Absorbance: λmax (water): 340 - 342, 225 - 228 nm
Presentation: Light Green Powder
Moisture content: <5%
Solubility: Soluble in water (5% w/v - clear, pale green solution).
Storage & Handling: Store at +4°C. Store Under Nitrogen. Protect from light.