Reagents, N

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  • One unit renders acid soluble 1 µg of denatured DNA (ssDNA) per minute at 37° C, pH 4.6.  This assay is based on the release of acid soluble products from ssDNA through hydrolysis.  One (1) µg of acid soluble products is equivalent to 0.022 A260 units. A solution in 30 mM Sodium…

  • Neutral protease (Dispase®) is designed for primary cell isolation and tissue dissociation. It is commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables…

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