Neutral protease (Dispase®) is designed for primary cell isolation and tissue dissociation. It is commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application. Expect a band approximately 36 kDa in size by western blot in the appropriate tissue or cell lysate. Specific conditions for reactivity should be optimized by the end user.