Nuclease S1 (Aspergillus oryzae sp.), 10ku, Liquid (sterile filtered)

One unit renders acid soluble 1 µg of denatured DNA (ssDNA) per minute at 37° C, pH 4.6.  This assay is based on the release of acid soluble products from ssDNA through hydrolysis.  One (1) µg of acid soluble products is equivalent to 0.022 A260 units. A solution in 30 mM Sodium Acetate, 50 mM Sodium Chloride, 1 mM Zinc Chloride and 50% (v/v) glycerol, pH 4.6.