Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCl, pH 7.4 (50mM) NaCl (150mM) Triton X-100 (1%) EDTA (5mM)

Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCL, pH 7.4 (50mM) SDS (2%)

G-Biosciences offers pre-packed 5 ml Immobilized Amino Acids resin in a column format.  Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking the amino or sulfhydryl group…

Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…

Sample buffer for protein extraction and purification of GST-fusion protein. Phosphate Buffer, pH 7.4 (10mM) Triton X 100 (1%) KCl (2.7mM) (2%) NaCl (140mM)

G-Biosciences offers G-Trap™ Immobilized Amino Acids resin in a  pre-packed column format.  Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking the amino…

Lysis buffer for extracting proteins from cells. 5X concentration Tris-HCL, pH 8.0 (0.5M) B-mercaptoethanol (100mM) EDTA (20mM) Add protease inhibitor according to your protocol

Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na₂HPO₄ (50mM) NaCl (300mM) Imidazol (10 mL)

Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na₂HPO₄ (50mM) NaCl (300mM) Imidazol (250mM)

Enzymatic lysis of gram-negative bacteria. Gram-negative bacteria are less susceptible than gram-positive bacteria to lysoyme and detergents. Enzymatic lysis of gram-negative bacteria requires the outer membrane to be permeabilized to expose the peptidoglycan layer to attack. The Bacillus…

Sample buffer for protein extraction and purification of GST-fusion proteins. Tris-HCl, pH 8.0 (50mM) NaCl (500mM) Triton X 100 (1%)

A 1M Phosphate solution containing BSA, NaCl, EDTA, and Azide. Product contains 50mL of 10X solution concentrate and can be diluted with distilled water to create 500mL or 1X solution. pH 7.4 (10mM) Stability >1 year at 4° C

Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na₂HPO₄ (50mM) NaCl (300mM) Imidazol (10 mL)

Quick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume

CellBuster-A Kit (Animal cell protein prep.) An efficient cell lysis medium for animal cells for preparation of total cellular proteins. Cell lysis facilitated by combination of proprietary detergent mixture. The kit contains enough reagents for 50 medium scale cultures. …

Sample buffer for HIS-TAG protein purification from mammalian cells. pH 8.0 Na₂HPO₄ (50mM) NaCl (300mM) Imidazol (20 mL) Tween-20 (0.5%)

Sample buffer for HIS-TAG protein purification from mammalian cells. pH 8.0 Na₂HPO₄ (50mM) NaCl (300mM) Imidazol (250mM) Tween-20 (0.5%)

The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an…

bio-World's GST-Tagged Protein Purification Buffer Kit consists of a set of all buffers necessary for OnePass™ GST-Tagged Recombinant Protein Purification System (Glutathione-Separopore ® conveniently prepacked spin columns for gravity flow chromatography) or bath…

FOCUS™ Cytoplasmic/Nuclear Protein Extraction (Thomas No. C994C79) Fractionation method is based on gentle lysis of the sample followed by centrifugation to collect and separate nuclei from the rest of the cytoplasmic proteins. Kit contains reagents necessary for fractionation and…

Quick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 25 ml column Concentrates 15 ml volume

The BIO-WORLD Abundant Protein Removal-Haptoglobin kit removes the high-abundant protein- Haptoglobin from human serum and plasma. The high affinity-based column contains separopore-matrix coupled with antibodies against the serum protein. The presence of abundant proteins in human serum and…

The bio-World Abundant Protein Removal-HSA kit removes the high-abundant protein- HSA from human serum and plasma. The high affinity-based column contains Separopore®-matrix coupled with antibodies against the serum protein. The presence of abundant proteins in human serum and plasma often…

"bio-World's OnePass ™ GST Tagged Protein Purification Kit consists of Glutathione Separopore ® (Glutathione reduced coupled to 4% cross-linked agarose beads), designed for the single-step affinity purification of proteins with a glutathione S-transferase…

The bio-World Abundant Protein Removal a-1-antitrypsin kit removes the high-abundant protein a-1-antitrypsin from human serum and plasma. The high affinity-based column contains Separopore® matrix coupled with antibodies against a-1-antitrypsin. The presence of abundant proteins in human…

DissolveAway is for solubilization of inclusion bodies in protein expression studies in bacteria Minimizes denaturation of proteins Increases efficiency and yield of your recombinant protein FoamFree ( Thomas No. C994H72 ) is recommended for use in large preparations

Common solution for protein and DNA isolation from yeast. Technical Information: LiCl (2.5M) Tris-HCl, pH 8.0 (50mM) Triton X 100 (1%) EDTA (62.5mM)

Foam Free, free-flowing dry powder, has been specifically developed for controlling foam in various biotechnology applications Prevents foaming during sonication or homogenization of cells and tissues for protein extraction, in hybridization solutions during rocking and shaking, and during…

Polyclonal antibodies (Rabbit) against polyHistidine are immobilized on Separopore ® (4% beaded agarose) to prepare a column for purification of His-tagged proteins using affinity chromatography. Bound protein is eluted using low-pH non-denaturing conditions. Kit includes all…

Uppa-PROTEIN-Concentrater Kit, Micro (Thomas No. C994C97) Protein solution as dilute as 1 ng/ml can be quantitatively concentrated by using the Universal Protein Precipitation reagent followed by solubilization and centrifugation through a Gel filtration spin column (provided). Concentrates…