Polyclonal antibodies (Rabbit) against polyHistidine are immobilized on Separopore® (4% beaded agarose) to prepare a column for purification of His-tagged proteins using affinity chromatography. Bound protein is eluted using low-pH non-denaturing conditions. Kit includes all components/buffers needed for quick and convenient purification.
- polyHis Antibody Separopore®, 2ml
- Empty columns, 2
- Binding buffer
- Elution buffer