Tryptic Soy Broth (TSB) is for growth of nonfastidious and moderately fastidious microorganisms (e.g., Neisseria, Listeria, and Brucella). The combination of soy and casein peptones supply organic nitrogen in the form of amino acids and polypeptides, making the medium highly nutritious. Sodium…

Soytone-based LB Broth (Miller formulation) is used for the growth of E. coli containing recombinant plasmids. Soytone is produced by enzymatic hydrolysis of defatted soybean meal/flour using papain (from papaya) and is therefore, an animal component–free source (ACF) of polypeptides, amino…

LB Luria Broth, Dry Media is powdered LB broth made according to the Luria formulation. The LB Luria formulation is used for the growth and maintenance of E. coli containing recombinant plasmids. This media is nutritionally rich, which allows for rapid growth. The Luria formulation contains…

LB Broth (Miller formulation) is used for the growth and maintenance of E. coli containing recombinant plasmids. This media is nutritionally rich, which allows for rapid growth. The Miller formulation is more commonly used than Lennox and Luria formulations, which both contain less sodium…

2X YT Broth is used for growth of recombinant E. coli and propagation of M13 bacteriophage in E. coli. Contains 1.6% (16 g/L) tryptone, 1.0% (10 g/L) yeast extract, 0.5% (5 g/L) sodium chloride.

Terrific Broth is a rich medium for cultivation of E. coli and is often used for growth to high density before plasmid purification. Tryptone and yeast extract provide nutrients, glycerol provides the carbon and energy source, and phosphate buffer helps stablize pH to prevent cell death. 1X…

LB Broth (Miller formulation) with Carbenicillin is used for the growth and maintenance of E. coli containing recombinant plasmids. LB Miller media is nutritionally rich, which allows for rapid growth. Carbenicillin is used to select for mutated or transformed cells that carry the…