LB Broth (Miller formulation) with Carbenicillin is used for the growth and maintenance of E. coli containing recombinant plasmids. LB Miller media is nutritionally rich, which allows for rapid growth.
Carbenicillin is used to select for mutated or transformed cells that carry the β-lactamase (bla) gene, which confers resistance to ampicillin (Amp or AmpR) and carbenicillin. Carbenicillin is a semi-synthetic ampicillin analog that does not allow growth of non-transformed, satellite colonies that sometimes grow in the presence of ampicillin due to extracellular secretion of lactamase. Typically, low-copy-number plasmids require less carbenicillin than high-copy-number plasmids. Note: 100 µg/mL carbenicillin is the most commonly used concentration.
Contains 1.0% (10 g/L) tryptone, 0.5% (5 g/L) yeast extract, 1.0% (10 g/L) sodium chloride, and carbenicillin (amount varies by part number).