Enzymes & Inhibitors, 5 g
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Iodoacetamide
MP BiomedicalsIodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins. Iodoacetamide can react with low molecular weight thiol compounds such as…
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5-Bromo-2'-Deoxyuridine
MP Biomedicals5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Measure DNA synthesis Label dividing cells Study cell signaling and processes that induce cell proliferation Used as a mutagen in…
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Collagenase Type II from Clostridium histolyticum
MP BiomedicalsCollagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…
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Lysozyme from Chicken Eggwhite
MP BiomedicalsLysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …
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ß-Mercaptoethylamine Hydrochloride
MP Biomedicalsβ-Mercaptoethylamine is a useful antioxidant .It is experimentally used as a radioprotective agent and It is also used to produce acute and chronic duodenal ulcers in rats and as an antidote to acetaminophen. β-Mercaptoethylamine derived from cysteine degradation is the simplest stable…
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Proteinase K
G-BiosciencesProteinase K (also protease K, endopeptidase K, peptidase K or Tritirachiumalkaline phosphatase) (EC 3.4.21.64) is a non-specifc, broad spectrum serine protease that is isolated from the saprophytic fungus Tritirachium album. Proteinase K is routinely used for the purification of target…
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Pepsin from Porcine Stomach Mucosa
MP BiomedicalsPepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…
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Cellulase from Aspergillis niger
MP BiomedicalsCellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose. …
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Cellulase (Onozuka R-10)
bioWORLDFrom Trichadema Viride. Enzyme mixture often used in combination with Macerozyme R-10 (M22010) for isolation of plant protoplasts. One unit of Cellulase will liberate 1.0 µmole of glucose from carboxymethyl cellulose. Lyophilized powder Optimum pH range: 4-5
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Lactase from Aspergillus oryzae
MP BiomedicalsLactase is an enzyme preparation produced by Aspergillus oryzae fermentation. Hydrolyzes Lactose Produces β-D-Galactose and a-D-Glucose One unit will hydrolyze 1.0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4.5 and 30°C …
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Lysozyme
bioWORLDEnzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.
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Lipase from Porcine Pancreas
MP BiomedicalsLipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. Subclass of esterases Catalyzes hydrolysis of emulsified esters of glycerol and long chain fatty acids One unit will liberate 100 µ moles fatty acid per hour…
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Catalase from Beef Liver
MP BiomedicalsCatalase is an oxidoreductase that catalyzes the conversion of hydrogen peroxide to water and oxygen. Activates the decomposition of hydrogen peroxide Natural antioxidant Used to study roles of reactive oxygen species in gene expression and apoptosis Hydrogen peroxide is a harmful…
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Naproxen
MP BiomedicalsPharmacological agent that reportedly may show activity through ability to inhibit prostaglandin biosynthesis. Non-selective cyclooxygenase (COX-1 and COX-2) inhibitor. Naproxen has been demonstrated to block Aβ fibril growth and form a complex with copper (II).
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Ficin
MP BiomedicalsFicin is a purified ficin preparation which is extracted from the latex of the fig tree Ficus glabrata. Ficin is classified as a thiol protease. Ficin hydrolyses the peptide bonds where the carbonyl group is from phenylalanine or tyrosine. When used in conjunction with other plants proteases,…
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a-Chymotrypsin 1X Crystallized from Bovine Pancreas
MP Biomedicalsα-Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine, and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. In addition to bonds involving aromatic amino acids, chymotrypsin catalyzes at a high…
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ABTS (2,2'-Azino-bis(3-ethylben
bioWORLDOne Component Microwell Peroxidase Substrate with excellent performance characteristics, good stability and wide working range. Develops blue-green color measurable between 405 nm and 410 nm.
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PMSF (Phenylmethylsulfonyl fluoride)
G-BiosciencesFeatures Synonyms: α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride CAS Number: 329-98-6 Molecular Formula: C₇H₇FOâ‚‚S Molecular Weight: 174.19 g/mol Activity: >99.9% …
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Cellulase from Trichoderma virde
MP BiomedicalsCellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose. …
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Lysozyme Type VI from Chicken Eggwhite
MP BiomedicalsLysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Lysozyme has been used for lysing E. coli and…
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Diamide
MP BiomedicalsDiamide is used as a thiol oxidizing agent. It has also been used to titrate protein glutathiolation to discriminate from other oxidative protein modifications. Diamide treatment increased protein glutathiolation in a concentration-dependent manner and had comparably little effect on…
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Esculin Monohydrate
MP BiomedicalsEsculin Monohydrate protects cells against DNA damage and reduces ROS levels. It is also a carcinogenesis inhibitor and displays vitamin P activity. Esculin is a coumarin glycoside that demonstrates antioxidant activitites and protection against chemically-induced carcinogenesis. Esculin is…
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Spermidine, Trihydrochloride
MP BiomedicalsSpermidine is a biogenic polyamine formed from putrescine. Essential in both normal and neoplastic tissue growth Plays an important role in the regulation of cellular proliferation and differentiation Forms stable compounts with nucleic acids Inhibits neuronal nitric oxide synthase…
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Trypsin Inhibitor from Soybean
MP BiomedicalsTrypsin inhibitor from soybeans is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges. Reversible serine protease inhibitor Inhibits factor Xa, trypsin, chymotrypsin, kallikrein and plasmin One unit will inhibit…
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Stigmasterol
MP BiomedicalsAnti-hypercholestrolemic compound. Anti-inflammatory and immune-modulating effects. Anticancer compound. Chemopreventive. Cytostatic. Cell growth inhibitor. Antimutagenic. Potent in vitro antagonist of FXR (farnesoid X receptor). DNA polymerase beta inhibitor. Potent antioxidant, hypoglycemic and…
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AEBSF (4-(2-Aminoethyl)benzenesulfon
bioWORLDInhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin and other related thrombolytic enzymes. Less toxic substitute for PMSF. Specific and irreversible inhibitor of serine proteases. Synonym: 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride pKb: 9.19 (Predicted) …
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Trypsin (bovine)
bioWORLDA serine peptidase that cleaves at internal peptide bonds on the carboxyl-side of arginine and lysine residues. USP Grade Activity (min.) at 25 C >2,500U/mg Chymotrypsin 5.0% Unit Definition: The amount of enzyme which causes an increase in absorbance of 0.003 per minute at 25 C…