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MP Biomedicals

Trypsin Inhibitor from Soybean

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Trypsin inhibitor from soybeans is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

  • Reversible serine protease inhibitor
  • Inhibits factor Xa, trypsin, chymotrypsin, kallikrein and plasmin
  • One unit will inhibit one trypsin BAEE unit. One trypsin unit causes an increase of absorbance at 253 nm of 0.001 per minute at 25°C

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.

After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.

Specific Activity: ≥10,000 BAEE units of inhibition/mg material

Key Applications: Cell culture

Application Areas: Cell biology

Product Type: Proteins, Enzymes & Peptides

Extinction Coefficient (E1%): E1% = 9.94 (280 nm, pH 7.6 buffer) (Lit.)

Presentation: Tan Powder

Format: Powder

Isoelectric point (pI): 4.5 (Lit.)

pH: Optimum pH = 7.0 (Lit.)

Solubility: Dissolves readily at 5 mg/mL in 0.067 M potassium phosphate pH 7.6 to give a clear colorless solution. Soluble in balanced salt solution (1 mg/mL) and serum-free medium.

Storage & Handling: Store at -20°C, desiccate.

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Product Detail
Thomas No.
C830V72
Mfr. No.
0210111325
CAS #
9035-81-8
Description
Trypsin Inhibitor from Soybean, 25mg
Packaging Size
25 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C830V73
Mfr. No.
0210111380
CAS #
9035-81-8
Description
Trypsin Inhibitor from Soybean, 100mg
Packaging Size
100 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C830V74
Mfr. No.
0210111383
CAS #
9035-81-8
Description
Trypsin Inhibitor from Soybean, 250mg
Packaging Size
250 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C830V75
Mfr. No.
0210111390
CAS #
9035-81-8
Description
Trypsin Inhibitor from Soybean, 500mg
Packaging Size
500 mg
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C830V76
Mfr. No.
0210111391
CAS #
9035-81-8
Description
Trypsin Inhibitor from Soybean, 1g
Packaging Size
1 g
Packaging Type
Bottle, Glass, Amber
list price/quantitytotal
$0.00
Thomas No.
C830V77
Mfr. No.
0210111394
CAS #
9035-81-8
Description
Trypsin Inhibitor from Soybean, 5g
Packaging Size
5 g
Packaging Type
Bottle, Glass
list price/quantitytotal
$0.00
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