Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
- Subclass of esterases
- Catalyzes hydrolysis of emulsified esters of glycerol and long chain fatty acids
- One unit will liberate 100 µ moles fatty acid per hour at pH 7.8 and 37°C using olive oil emulsion as substrate.
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate). Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Specific Activity: >12,000 u/g solid
Key Applications: Resolution of chiral compounds, Transesterification production of biodiesel.
Application Areas: Molecular biology
Product Type: Proteins, Enzymes & Peptides
Extinction Coefficient: (E1%) E1%280 = 13.3(Lit.)
Presentation: Tan Powder
Format: Powder
Isoelectric point (pI): Lipase A = 4.9 and Lipase B = 5.0 (Lit.)
NOTES: Activators: Ca2+ is required for activity [Sr2+ and Mg2+ are less effective activators. Inhibitors: Versene, Zn2+, Cu2+, Hg2+, iodine, PCMB. DFP does not inhibit. Stabilizers: DFP may be used to stabilize impure preparations containing proteinases in solutions.
Solubility: Partly soluble in water and insoluble in alcohol.
Storage & Handling: Store at -0°C.