Today’s media routinely covers forensic stories of criminals captured and innocents released using the powerful DNA fingerprinting technique. This kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a…

A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin.   The solution…

Microorganisms, such as bacteria and fungi, are all around us. This kit allows students to collect samples from the world around them and grow the microorganisms so that students can visualize the microorganisms.  This teaches students how to plate and grow microorganisms.   …

This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate and distinguish proteins by…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

Hydrophobic chromatography is based on the fact that protein molecules can have extensive hydrophobic regions.  These hydrophobic regions, in media favoring hydrophobic interactions, such as an aqueous solution with high salt concentration, can bind to hydrophobic ligands coupled to an…

Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques.  The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA. …

This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification.  The kit contains the reagents to allow students to: Equilibrate a DEAE agarose column Load a protein sample on the column (amylase, lysozyme or a mixture of…

Water pollution is a major problem throughout the world, raising severe concerns about health and sanitization issues.  A common problem in polluted water is the presence of bacteria, particularly coliform bacteria from fecal matter, which can directly result in disease, as well as act as a…

Proteins are the building blocks of life and there are estimated to be almost 1 million different proteins in a normal animal cell.  Each protein has very different and unique physical properties. The Physical Properties of Proteins kit is a lab activity that enables students to investigate…

This Biotechnology Basics™ by Ellyn Daugherty is designed to teach students the basic principles of measuring small volumes (<500µl) using micropipettes. The kit is supplied with all the reagents to allow 8 groups of 4 students to learn about P-1000, P-200 or P-100 and P-20 or P-10…

Lab activity that teaches use of specific lock-key matching properties of antigen-antibody for detection and characterization of antibody or antigenic proteins in complex samples. One such widely used method is known as Western Blot analysis. Western Blot analysis involves electrophoresis…

This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach students about RFLP (restriction fragment length polymorphisms) and its use in crime scene investigations. Students will use restriction enzyme digestions of victim, suspect and crime scene DNA and then visualize the…

The Quantitative Precipitin Technique is a simple technique that is routinely used in the analysis of antibody and antigen interactions and for the estimation of the antibody or antigen content in a sample. The technique is based on the interaction of antibody and antigen to form a large protein…

Genomic DNA isolation is a crucial technique in molecular biology, genetic manipulation and biotechnology.  To carry out these exciting and innovative sciences, researchers require an original source of DNA, which is easy to acquire as it is found in every living organism.  Genomic DNA is…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.    The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches…

Size exclusion chromatography (SEC), also called gel filtration chromatography or gel-permeation chromatography (GPC), uses porous particles to separate molecules of different sizes.  It is generally used to separate biological molecules and to determine molecular weights and molecular weight…

An important and essential tool in protein analysis is electrophoresis.  Protein electrophoresis allows researchers to separate complex protein samples by their molecular size and/or net charge on polyacrylamide gels.  The proteins can be subsequently visualized with various protein…

A comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development…

Our Bacterial Gram Staining kit is supplied with three strains of bacteria and all the necessary prealiquoted components to carry out the Gram staining to determine Gram positive and negative bacteria. The Gram staining method was first described in 1844 by the Danish bacteriologist Hans…

Dialysis is a routinely used technique in research laboratories to “change” the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as…

This lab activity teaches the use of highly specific matching properties of antigen-antibody for detection of antigenic or antibody proteins in complex samples.   One such immunodetection method is widely known as Dot Blot. The method involves applying small volumes of antigens or test…

A comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development…

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes.  This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic…

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.  The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students…

This kit is designed to teach 8 groups of four students the principles of cheese making.   The kit contains the reagents to allow students to: Set up a controlled experiment & collect numerical data Determine which curdling agent tested produces cheese at the fastest rate …

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)   The kit contains the reagents to allow students to: Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates Set up a PCR negative…

This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach the basic principles of a gel box, specifically horizontal agarose gel electrophoresis. The kit is supplied with all the reagents necessary to conduct 4 gel box science experiments, each using a gel box (not supplied). …

This kit is designed to teach 8 groups of four students the principles of Western blotting. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate proteins by electrophoresis …

Zymography is an electrophoretic technique that includes a substrate copolymerised with the polyacrylamide gel for the detection of enzymes and their activity. Samples are prepared without denaturing the active enzymes present in the samples. Following electrophoresis, the gel is placed in an…

This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution to create standards of known concentration Bind known…

Nucleic acids (DNA and RNA) and proteins are routinely resolved by electrophoresis, which separates molecules based on size and/or charge. Following electrophoresis, the molecules need to be extracted from the electrophoresis medium for use in downstream applications and experiments.…