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Immunoaffinity Chromatography Kit

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A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin.


The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction.


In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilized in research laboratories.


Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.



  • Teaches antibody:antigen precipitation.
  • Hands-on activity to precipitate antigen and antibody complex.
  • Quantitative assessment of antibody concentration.



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Immunoaffinity Chromatography, For 6 groups of 4-5 or 24-30 students.
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