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The rAmylase Project: Confirming the Amylase Gene Using PCR Kit

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This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)


The kit contains the reagents to allow students to:

  • Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates
  • Set up a PCR negative control 
  • Carry out the PCR reaction
  • Load, separate, and visualize PCR DNA products on an agarose gel
  • Confirm a suspected origin of the amylase gene in nature



  • For 8 groups of 4 students 
  • Requires thermocycler/ PCR machine, agarose electrophoresis equipment including UV transilluminator (light box) and micropipettes and tips



  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function



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The rAmylase Project: Confirming the Amylase Gene Using PCR, 8 x 4 student groups
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