This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)
The kit contains the reagents to allow students to:
- Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates
- Set up a PCR negative control
- Carry out the PCR reaction
- Load, separate, and visualize PCR DNA products on an agarose gel
- Confirm a suspected origin of the amylase gene in nature
Features
- For 8 groups of 4 students
- Requires thermocycler/ PCR machine, agarose electrophoresis equipment including UV transilluminator (light box) and micropipettes and tips
Applications
- HS-LS1: From Molecules to Organisms: Structures and Processes
- LS1.A: Structure and Function