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The rAmylase Project: Direct ELISA of Bacterial a-Amylase Kit

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This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).


The kit contains the reagents to allow students to:

  • Conduct a serial dilution of a stock amylase solution to create standards of known concentration
  • Bind known and unknown amylase samples to ELISA wells
  • Wash and recognize amylase protein with an enzyme-labeled antibody
  • Detect amylase-bound, enzyme-labeled antibody with a colored substrate
  • Quantify the amount of amylase protein in unknown samples by using a standard curve of known amylase concentrations



  • For 8 groups of 4 students 
  • Requires micropipettes and tips
  • Optional requirement for a plate reader



  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function

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The rAmylase Project: Direct ELISA of Bacterial a-Amylase, 8 x 4 student groups
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