ACD (Acid Citrate Dextrose) Solution A andACD (Acid Citrate Dextrose)Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma. Features Solution A: 38mM citric acid, 107mM sodium citrate, 136mM dextrose Solution B: 23mM citric acid,…

PBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X:  8mM NaH₂PO_4, 150mM NaCl, 3mM KCl, 2mM KH₂PO4 (pH 7.4) 10X: 80mM NaH₂PO₄ 1.5M NaCl,…

Features   Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol Linear Formula: NH₂C(CH₂OH)₃ Molecular Weight: 121.14 CAS Number: 77-86-1

Phosphate buffers are buffer solutions commonly used in biological research. They are water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH. Competitive remarks and statements Feature statements Application statements Features Useful pH…

The Trypsin Digestion Mix provides optimal buffered conditions for in gel trypsin digestion of proteins. Suitable for 100 protein spots.

Synonyms: TE Buffer, Tris-EDTA Buffer, T10E1 Buffer The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. The TE Buffer [10X] is offered in 0.5 and 1L…

Tris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels Features Tris-Acetate/SDS running buffer is compatible with Tris/Acetate gels Separate high molecular weight proteins (36-400 kDa) under…

Features SDS-PAGE Running Buffer (10X) Compatible with Tris-Glycine gels Separate proteins in the range 6-200 kDa under denaturing conditions 1 X concentration: 25mM Tris Base, 192 mM Glycine, 0.1% SDS Applications Tris-Glycine/SDS Running Buffer [10X] is compatible…

Features Synonym: Acetic acid sodium salt CAS#: 127-09-3 Molecular Formula: C₂H₃O₂Na Molecular Weight: 82.03

Features 50mM Tris.HCl (pH7.5), 100mM NaCl, 8mM MgSO₄, 0.01% Gelatin

JAW™ Tris-HEPES-SDS Buffer Packs are ready to use dry-blend powder pouches used to make running buffer for SDS-PAGE with Tris-HEPES gels Features : Each pack when dissolved in deionized water and made to final volume of 500 ml makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8 Come in…

For growing cells and for competent cells preparation Features 270mM Sucrose, 10mM Tris-HCl, ph 7.5, 1mM MgCl₂

 A 10X Concentrated solution of Tris Buffered Saline with Tween® 20.    The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5.   The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5.   TBST…

Features 0.25M Tris, 1.92M Glycine, pH 8.3

Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. Features 2% Ficoll 400 0.3M sodium chloride 2% PVP…

TE Buffer [1X], pH 8.0, Low EDTA (Tris-EDTA; 10mM Tris base, 0.1mM EDTA) are available in different sizes. Other concentrations of TE Buffer are offered, including a 100X concentration and a 10X concentration. 10X and 100X regular TE Buffers are also available Features Low EDTA…

Features Tris-Glycine Native Running Buffer [10X]

Features 0.9M Tris-borate, 0.01M EDTA (pH8.3)

Features 2M Tris-Acetate, 0.05M EDTA (pH8.3)    

The SDS-PAGE Sample Loading Buffers are suitable for loading protein samples on to the SDS-polyacrylamide gels. The buffers are provided in 2X and 6X concentrations containing Tris-HCl, glycerol, SDS and bromophenol blue (BPB) in recommended concentrations and is stable at room temperature. …

Tris-EDTA-NaCl Buffer solution at pH 8.0. A customer enquired about this buffer to make as custom and we also added as a new product. Features Precision in concentration and pH Guaranteed reproducibility Applications As a buffering mix

Buffer for DNA/RNA extraction and purification. Features 8% w/v sucrose, 5% v/v Triton® X-100, 50mM EDTA, 50mM Tris-HCl, pH 8.0

Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands. For the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies,…

A selection of 12 x 25ml molecular biology relevant buffers. These buffers are routinely used in molecular biology and are all DNase and RNase free. The buffers supplied are listed below. Features Molecular Biology Universal Kit Ammonium acetate [5M] Calcium chloride [1M] …

JAW™ Borate Buffer Packs are dry blend powder pouches used to make borate buffer which is used in protein modification procedures that require amine free and alkaline pH conditions. Features : Each Pack is dissolved in 500 ml deionized water to get 50 mM borate buffer, pH8.5 Come…

Saline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features …

For DNA/RNA Extraction and Purification Features 3M NaCl, 165mM Tris-HCl, pH 8.0, 45mM EDTA, pH 8.0, and 16.4% Tween® 20

Based on Efficient ™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting. Supplied as 1 liter of a 5X concentrated solution.

Chaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…

Features   Synonym: Disodium hydrogen phosphate CAS#: 7558-79-4 Molecular Formula: Na₂HPO₄ Molecular Weight: 231-448-7

Features Borate Saline Buffer [2X] 400mM Boric acid, 300mM NaCl, 500mM EDTA

G-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …