G-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …

Zymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is…

Tris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels Features Tris-Acetate/SDS running buffer is compatible with Tris/Acetate gels Separate high molecular weight proteins (36-400 kDa) under…

Saline-Sodium Citrate HybridizationBuffer [20X] controls stringency during the washing steps in Southern blotting, insitu hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features 0.3M trisodium…

JAW™ Tris-HEPES-SDS Buffer Packs are ready to use dry-blend powder pouches used to make running buffer for SDS-PAGE with Tris-HEPES gels Features : Each pack when dissolved in deionized water and made to final volume of 500 ml makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8 Come in…

G-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.The buffers are ideal for the purification of antibodies from Protein A , Protein G and Protein A/G resins. IgG Binding…

Features   Synonym: Monopotassium phosphate CAS#: 7778-77-0 Molecular Formula: KH₂PO₄ Molecular Weight: 136.09

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification…

Features Synonym: Acetic acid sodium salt CAS#: 127-09-3 Molecular Formula: C₂H₃O₂Na Molecular Weight: 82.03

Features 0.9M Tris-borate, 0.01M EDTA (pH8.3)

Features 2M Tris-Acetate, 0.05M EDTA (pH8.3)    

Based on Efficient ™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting. Supplied as 1 liter of a 5X concentrated solution.

Our RBC Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, when used as directed. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood…

Features Synonym: Natron CAS#: 144-55-8 Molecular Formula: NaHCO3 Molecular Weight: 84.01

Features Tris-Glycine Native Running Buffer [10X]

Chaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…

Features 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available

Features   Synonym: Disodium hydrogen phosphate CAS#: 7558-79-4 Molecular Formula: Na₂HPO₄ Molecular Weight: 231-448-7

ACD (Acid Citrate Dextrose) Solution A andACD (Acid Citrate Dextrose)Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma. Features Solution A: 38mM citric acid, 107mM sodium citrate, 136mM dextrose Solution B: 23mM citric acid,…

Isoelectric Focusing Cathode Buffer, pH3-7 Features pH 3-7

Acetate Buffer at pH 5.0 and 5.5 are supplied as ready to use solutions that eliminates the calculation, formulation and weighing errors. Features Precision in pH Guaranteed reproducibility

A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na₂HPO₄, 1.5M NaCl, 20mM KH₂PO₄, 30mM KCl, 0.5% Tween® 20, pH 7.4.   PBST is commonly used as a wash solution for Western blot…

Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands. For the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies,…

The SDS-PAGE Sample Loading Buffers are suitable for loading protein samples on to the SDS-polyacrylamide gels. The buffers are provided in 2X and 6X concentrations containing Tris-HCl, glycerol, SDS and bromophenol blue (BPB) in recommended concentrations and is stable at room temperature. …

Features 100mM Tris.HCl (pH 8.0), 10mM EDTA, 1M NaCl Synonym: TEN Buffer

PBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X: 8mM NaH₂PO_4,150mM NaCl, 3mM KCl, 2mM KH₂PO4(pH 7.4) 10X: 80mM NaH₂PO₄ 1.5M NaCl, 30mM KCl, 20mM…

For DNA/RNA Extraction and Purification Features 3M NaCl, 165mM Tris-HCl, pH 8.0, 45mM EDTA, pH 8.0, and 16.4% Tween® 20

Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. Features 2% Ficoll 400 0.3M sodium chloride 2% PVP…

Features   Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol Linear Formula: NH₂C(CH₂OH)₃ Molecular Weight: 121.14 CAS Number: 77-86-1

TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. Suitable for 10 liters of 1X TE Buffer Features Synonyms: TE Buffer,…

Features 0.25M Tris, 1.92M Glycine, pH 8.3

For growing cells and for competent cells preparation Features 270mM Sucrose, 10mM Tris-HCl, ph 7.5, 1mM MgCl₂