Tissue Culture Media

This 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the...

Technical Advantage:  Lower documented Endotoxin content then offerings from other major suppliers. Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO2 atmospheric conditions.  This formulation is produced without sodium...

Ham's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids.  This medium is also suitable for carcinoma cells, rat skeletal myoblasts,...

Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN...

Growth Promotion Test Suspensions provide a quality control challenge to each batch or lot of medium, ensuring its growth promoting qualities, whether it is prepared internally from basic ingredients or purchased commercially prepared. Inoculate each batch or lot of medium, directly without...

The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and...

Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the...

Ampicillin is used to select for mutated or transformed cells that are ampicillin resistant (Amp or Amp R ) [i.e., carry the ß-lactamase ( bla ) gene]. Typically, low-copy-number plasmids require less ampicillin than high-copy-number plasmids. In some situations (e.g., if extracellular lactamase...

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of...

McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. 

Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN...

Specialized for use in insect cell culture Poloxamer 188 is a surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring. It is a non-ionic detergent with an average molecular weight of 8400Da consisting of a central...

Dehydrated culture media Refrigerate, store at 2° to 8°C BD products are intended for sale/shipment within the US only. International customers should contact their local BD distributor to place orders for BD products.

This modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate...

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications for a range of cell culture applications. Applications DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM...

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow normal...

Ham's F-10 is a classical media designed to support the growth of a variety of cell types, including HeLa, Chinese Hamster Ovary (CHO), primary cells from tissue explants and various types of human diploid cells. 

Laminin (LM), a major component of basement membranes, is a multifunctional glycoprotein that is used as a substrate to culture and maintain differentiated function of a wide variety of cells. Laminin has been shown in culture to stimulate neurite outgrowth, promote cell attachment, chemotaxis, cell...

Corning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications.

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence...

CDC Anaerobe 5% Sheep Blood Agar is used for the isolation and cultivation of fastidious and slow growing, obligately anaerobic bacteria from a variety of clinical and nonclinical materials. It also supports good growth of most aerobic, facultatively anaerobic and microaerophilic bacteria if...

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend pelleted cells...

Trace Elements A provides copper, zinc, iron, and selenium. Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB 301....

PBS (Phosphate Buffered Saline) Powdered: PBS (Phosphate Buffered Saline) Powdered. 1 PK makes 1 L of 10x concentrated PBS or 10 liters of 1 x PBS solution. This PBS formulation is recommended for use in immunohistology protocols. Each package makes 1 L of 10 concentrated PBS or 10 liters of 1 x...