Quality Biological Buffers

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

Tris HCl, 10X pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications Tris buffered saline is useful for the following: as a biological diluent buffer, as a buffer for antibodies, as a wash...

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989). The purpose of this reagent is to insure that the RNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. Contains two dyes, bromphenol blue &...

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol Red Applications DPBS can be used to provide a buffer...

A hybridization buffer specifically formulated for use with nylon membranes. It does not contain Denhardt’s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. Formulated for Nylon membrane Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested...

SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front. Lane Marker Sample Buffers may be...

Sodium Chloride, 0.85% is a saline solution at 0.85% prepared with water used for cell culture. Made with cell culture grade water The sodium chloride is a source of chlorine and sodium necessary to the production of components like sodium carbonate, sodium hydroxide or chlorate ions. Applications...

Protein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis. Contains 1% SDS Filtered through a 0.22 µm membrane...

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its...

TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus or reporter...

Tris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris HCl...

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research. pH 7.2 – 7.6 Applications HEPES 1M is used as a buffer alternative from sodium...

DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer....

TRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X). Applications Tris Glycine Transfer Buffer, at a 1X concentration with the addition of 20% methanol, is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane – i.e. Western blotting or...

Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its...

TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. DNase, RNase and Protease tested. Applications TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be...

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Without EDTA Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid...

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,...

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents,...

Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and...

Tris HCl 2 M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris...

Normal Saline’s isotonic solution is used as a general laboratory diluent. With 9 g/L sodium chloride For non clinical use Applications Normal Saline can be used in the following microbiology applications: solvent, washing agent for cells/bacteria/specimen, buffer in aseptic techniques and plaque...

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...