Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface.
Without EDTA
Applications
Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.
Recommendation
Do not add calcium or magnesium.
Prolonged exposure can negatively affect cell viability. Be aware of exposure time.
Storage Conditions: -10°C to -20°C
Shipping Condition: on dry ice
Sterile Filtered
Performance Tested
Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health
Harmonization Code: 3822.00.5090
ECCN: EAR99
Max Shelf Life: 24 months