PCR Reagents And Kits

Choice Taq Mastermix is a 2X reaction mixture. It is ready-to-go and it only requires addition of template, primers, and water. Choice Taq Mastermix diminishes the amount of time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is also ensured by...

Storage: −20°C UNSPSC Code: 12352200 Components: FastStart High Fidelity Enzyme Blend, in storage buffer 5 U/μl; FastStart High Fidelity Reaction Buffer, with 18 mM MgCl₂ 10x concentrated; FastStart High Fidelity Reaction Buffer, without MgCl₂ 10x...

Components Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX. General description 2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments. The EagleTaq...

This reagent is designed for use with the MasterPure™ kit protocols only.

Inactive at room temperature Increased specificity This is the Hot-Start version of Choice-Taq™ DNA Polymerase that requires a pre-activation step at 95°C for 5 minutes in order to achieve a fully functional polymerase enzyme. The inactivity of the enzyme at room temperature increases the...

Storage: −20°C UNSPSC Code: 12352200 Components: Expand High Fidelity Enzyme mix; Expand High Fidelity Buffer, with 15 mM MgCl₂ 10x concentrated; Expand High Fidelity Buffer, without MgCl₂; MgCl₂ Stock Solution 25 mM; PCR Nucleotide Mix RIDADR: NONH for...

EpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity

Storage: −20°C UNSPSC Code: 41106300 Components: GC-RICH Enzyme Mix, in storage buffer; GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl₂ and DMSO 5x concentrated; GC-RICH Resolution Solution 5 M; MgCl₂ Stock Solution 25 mM; Water, PCR Grade RIDADR: NONH for all modes of...

MyTaq™ HS Red DNA Polymerase is a new generation of antibody-mediated hot-start enzyme, engineered for highly specific and efficient amplification from even the most challenging templates. MyTaq HS remains inactive at room temperature allowing for convenient reaction set-up, thereby reducing...

Bioneer’s AccuPower PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq” Top DNA Polymerase for the fastest...

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

TransforMax™ EC100™ Electrocompetent E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency.

Hot-Start Taq Mastermix™ is the heat activated version of Denville's 2X, ready to go reaction mixture. It is inactive at room temperature and only requires addition of template, primers, and water. A 5 minute pre-heat step at 95°C is required to successfully carry out primer extensions. The Blue...

ClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by...

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that...

Azura SmartStain™ is a safe alternative to EtBr that exhibits excitation peaks at 290nm & 490nm, allowing it to be used with UV and blue light. SmartStain™ PS (Pre-Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The...

Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures

The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.

A convenient mastermix containing a new generation of hot-start polymerase that delivers improved specificity, yield, speed and robustness when amplifying targets from any template. Product Highlights Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA,...

Ready-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.

KOD DNA Polymerase* (formerly KOD HiFi DNA Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase (Nishioka 2001). KOD is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield (Takagi 1997). The enzyme’s 3'?5'...

BioMix™ Red is a complete ready-to-use 2x reaction mix containing an ultra-stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel. Product Highlights Convenient format - pre-mixed, pre-optimized 2x solutions Versatile -...

KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'?5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start combines the high fidelity, fast extension speed, and outstanding...

Respiratory pathogens cause acute local and systemic disease, such as pneumonia. Due to the similarity of diseases caused by many bacteria or fungi, diagnosis based on clinical symptoms alone is difficult. Identification of potential causative agents provides data to aid the physician in determining...