ROCHE Expand™ High Fidelity PCR System, dNTPack

Storage: −20°C

UNSPSC Code: 12352200

Components: Expand High Fidelity Enzyme mix; Expand High Fidelity Buffer, with 15 mM MgCl₂ 10x concentrated; Expand High Fidelity Buffer, without MgCl₂; MgCl₂ Stock Solution 25 mM; PCR Nucleotide Mix

RIDADR: NONH for all modes of transport

Features and Benefits:
• Use one enzyme for all applications. Enjoy high yield, fidelity, and flexibility.
• Detect PCR products previously undetectable and avoid false negatives.
• Achieve successful results from small quantities of template DNA.

Application:
The Expand^™ High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length for:
• PCR
• RT-PCR
For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.

General description:
Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. The Expand High Fidelity PCR System is composed of a special enzyme mix containing thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA Polymerase. T/A cloning is recommended.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Quality:
Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1kb amplified product) and the tPA gene (4.8kb amplified product).

Legal Information:
Expand is a trademark of Roche