EICOSANOIDS-EIA (Enzyme Immunoassay)

Maresins (macrophage mediators in resolving inflammation) are members of a class of potent anti-inflammatory and pro-resolving mediators that are synthesized by macrophages in the presence of DHA. MaR1 is produced via oxidation of endogenous DHA by 12-LO. MaR1 has been shown to reduce neutrophil...

15(S)-HETE is produced from arachidonic acid by the enzyme 15-LO. In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes. It may contribute to allergic rhinitis, has anti-inflammatory properties, and acts as a vasoconstrictor. Cayman’s 15(S)-HETE ELISA is...

The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm. Inhibition of COX activity by a variety of selective and nonselective inhibitors shows...

The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and...

The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. Inhibition of COX activity, measured by...

PGF2α is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. The majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. Like all of the primary PGs, PGF2α has a very short half-life in the general circulation....

Fluprostenol is a metabolically stable analog of PGF2α with potent FP receptor agonist activity. The isopropyl ester of fluprostenol has been approved for use as an ocular hypotensive drug for the treatment of glaucoma and is sold under the Alcon trade name Travoprost. Acting as a prodrug,...

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also...

Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction...

The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific antiserum that binds to all the major prostaglandin compounds. This assay includes...

This assay was developed for screening applications in which the relative amount of PG production for a large number of cell culture samples must be determined. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given...

LTB4 is synthesized from arachidonic acid by the combined action of 5-LO and LTA4 hydrolase. LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release,...

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The...

Prostaglandin I (Prostaglandin I2; PGI2) is a potent vasodilator and inhibitor of platelet aggregation that is formed from arachidonic acid primarily in the vascular endothelium and renal cortex by sequential activities of cyclooxygenase (COX) and PGI2 synthase. It is non-enzymatically hydrated to...

For the quantification of TXB₂ in urine, serum, tissue culture supernatants, and other sample matrices Sensitivity (80% B/B0): 5 pg/ml Assay Range: 1.6-1,000 pg/ml Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and...

Prostaglandin D2 (PGD2) is biosynthesized in the brain by soluble, glutathione-independent lipocalin-type PGD2 synthase. PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular,...

Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously...

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC4, LTD4, and LTE4 are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC4 and LTD4 are potent mediators of asthma and hypersensitivity....

The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell...

This PGD2-MOX ELISA is based on the conversion of PGD2 to a stable MOX derivative. Treatment of the sample with MOX HCl converts PGD2 into PGD2-MOX, preventing its further chemical degradation. The antiserum used in the assay was developed using conjugates of this derivative and is very specific for...

tetranor-PGDM is a major metabolite of PGD2 found in human and murine urine. Normal levels of tetranor-PGDM in human and murine urine are 1.5 ng/mg creatinine and 8.1 ng/mg creatinine respectively. Cayman’s tetranor-PGDM ELISA is a competitive assay that can be used for quantification of...

Cayman’s sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s with the exception of cPLA2. Upon hydrolysis of the thioester bond at the sn-2...

Cayman’s FABP4 Ligand Binding Assay provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a detection reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will...

PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α. The Cayman’s...