Quality Biological

Nuclease and Endotoxin-Free, Sterile DEPC-treated water is certified nuclease-free and supplied in four bottles containing 1 L each to ensure sterility and inactivation of DEPC. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity, protease....

M9 CA Media contains the essentials for bacterial species to grow. Glucose as a carbon source and casamino acids as a nitrogen source. Important components of M9 CA medium are the carbon source from glucose, a nitrogen source from ammonia chloride, and electrolytes from sodium, chloride, potassium,...

Protein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis. Contains 1% SDS Filtered through a 0.22 µm membrane...

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its...

Amphotericin B, Penicilllin, Stretomycin Mix is useful for reducing the chance of microbial contamination. Convenient mixture of the antibiotics and antifungal agent in one bottle. 100X concentration, just dilute 1:100 in the culture medium Recommended for use in cell culture applications at 10...

SOC medium is a ready-to-use solution, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. Without magnesium chloride, needs to be added before use. Autoclave sterilized Applications SOC is a microbial growth medium used for the...

TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus or reporter...

Tris HCl 2M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).Tris HCl...

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research. pH 7.2 – 7.6 Applications HEPES 1M is used as a buffer alternative from sodium...

Sodium acetate is a widely used reagent in molecular biology applications. It is used as a buffer in conjunction with acetic acid, in the buffering range of pH 3.6 – 5.6. Applications Sodium acetate is used in the purification and precipitation of nucleic acids, protein crystallization, staining of...

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With Stabilized L-glutamine Applications DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM was originally developed as a richer version...

DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer....

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production. Has 220% more peptone and 300% more yeast extract than LB Autoclave sterilized Applications Super Broth is the best commonly used E. Coli growth medium for plasmid production. Super...

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement...

TRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X). Applications Tris Glycine Transfer Buffer, at a 1X concentration with the addition of 20% methanol, is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane – i.e. Western blotting or...

LB Agar 1.5% Lennox provides all nutritional requirements of these organisms. LB Agar 1.5% Lennox contains half the sodium chloride level of the Miller formulation of LB Agar. Just autoclave to melt and pour. Applications LB Agar 1.5% Lennox is nutritionally rich. It was developed by Lennox for...

Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications Tris...

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its...

TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. DNase, RNase and Protease tested. Applications TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be...

Hanks' Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbohydrate as an energy source. The HBSS without Ca and Mg formulation...

Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or growth...

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Without EDTA Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid...

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,...

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents,...