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Hyacinth BMT activator (also known as 5-Benzylmercapto-1H-tetrazole or BTT) demonstrates important advantages in the syntheses of oligonucleotides: Coupling efficiencies of 99% with impeccable quality Lower percentage of n - 1 sequences Dramatic reduction of coupling times to under 3 minutes in RNA...
CentriPure Gel Filtration Columns are designed for rapid desalting and efficient removal of small molecules (dyes, salts, buffers, biotin, haptens, etc.) from proteins and nucleic acids.
For each synthesis cycle, up to 1 to 2 % of free 5’-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent „Capping“ step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain...