Cayman Chemical

The product of nitration of linoleate by NO-derived reactive species; activates PPARγ (Ki = 133 nM), inducing CD36 expression in macrophages, adipocyte differentiation, and glucose uptake; can also be metabolized to produce nitrite derivatives which in turn form NO, leading to increased cGMP...

kb NB 142-70 is a selective inhibitor of protein kinase D (PKD) with IC₅₀ values of 28.3, 58.7, and 53.2 nM for PKD1, 2, and 3, respectively. It has been shown to inhibit prostate cancer cell migration and invasion, as well as reduce wound healing in vitro.

An affinity probe designed to allow linoleic acid to be detected in complexes with protein-binding partners such as fatty acid binding proteins.

A lincosamide antibiotic effective against Gram-positive bacteria that acts by inhibiting bacterial protein synthesis via binding to the 50S subunit of the ribosome.

An aldehyde product of the oxidative deamination of dopamine that is toxic to neurons.

A natural sesquiterpene lactone which inhibits the growth of the promastigote form of L. amazonensis (IC₅₀ = 3.6 μg/ml); induces apoptosis in cancer cells, at least in part by inhibiting NF-κB- and STAT-mediated anti-apoptotic gene transcription; directly binds the pattern...

Arachidonic acid is converted by microsomal CYP450 enzymes to a variety of epoxides, ω-1 and ω-hydroxylated compounds via what is known as the epoxidase pathway. MS-PPOH is a selective inhibitor of the epoxygenation reactions catalyzed by specific CYP450 isozymes. MS-PPOH inhibits the...

A cell culture supplement that can sustain self-renewal and pluripotency of mouse embryonic stem cells at 10 μM for > 30 days.

Inhibits ALK5- (IC50 = 47 nM), ALK4- (IC50 = 129 nM), and ALK7-dependent activation of downstream SMAD2 and SMAD3 and TGF-β–induced MAP kinase pathway components without altering ALK1-3 or ALK6-induced SMAD signaling.

Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative protocol....

An autophagy inhibitor, inducing cell death in human breast cancer Bcap-37 cells grown in glucose-free media (EC₅₀ = ~1.25 µM); promotes the degradation of Vps34 PI3K complex by inhibiting the deubiquitinating enzymes USP10 and USP13 (IC₅₀s = 0.58 and 0.69 µM,...

A SAHA derivative with a benzophenone crosslinker and an alkyne tag to be used for profiling HDAC activities in proteomes and live cells; labels HDAC complex proteins both in proteomes at 100 nM and in live cells at 500 nM; IC50 = ~3 μM for inhibition of HDAC activity in HeLa cell nuclear lysates...

10-Pyrene-PC is a substrate for all PLA2s with the exception of cPLA2 and PAF-AH. Upon phospholipid hydrolysis, 10-pyrenyldecanoic acid is produced from 10-pyrene-PC. The monomeric 10-pyrenyldecanoic acid exhibits fluorescence (excitation 345 nm, emission 395 nm), allowing quantitation of...

A bifunctional cross-linking reagent reactive toward primary amines; is membrane-permable and commonly used to cross-link proteins in intact cell.

Features Each vial contains a mixture of standards packaged in amber ampules purged with argon >1 ml provided at the concentration specified in the insert Ideally suited for use as an internal standard in mass spectrometry applications This mixture contains deuterated versions of selected...

A sugar sulfamate that exhibits potent anticonvulsant activity, completely blocking seizures (ED50 = 39 mg/kg orally) in mice in the maximal electroshock seizure test; the drug form Topamax® is used to treat seizures associated with epilepsy and to prevent migraine headaches.

A bifunctional photoreactive agent for crosslinking nucleic acids; intercalates into double-stranded regions in DNA or RNA and, upon UV irradiation, forms covalent adducts with pyrimidine bases; used to study different types of RNA from diverse organisms, including viruses.

An analog of alliin found in some cruciferous vegetables that can be converted through alliinase activity into a volatile thiosulfinate.

The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and...

CWHM12 is an analog of RGD peptide (Item No. 14501), a tripeptide that inhibits integrin-ligand interactions in studies related to cell adhesion, migration, growth, and differentiation. CWHM12 selectively inhibits αv integrins (IC₅₀s = 1.8, 0.8, 61, 1.5, and 0.2 nM for...

A cell-permeable compound that maintains long-term mouse ESC pluripotency; allows ESCs to form embryoid bodies that continue to express markers of pluripotency when used at 4 µg/ml with Wnt3a; blocks the interaction of Nkd with a subunit of PP2A.

An internal standard for the quantification of 9-PAHSA by NMR or GC- or LC-MS.

A substrate of the CYP450 isoform CYP2C19, also known as mephenytoin 4-hydroxylase; can be used to screen for mutations in CYP2C19 by assaying its metabolites in urine.

A stereoisomer of the antitumor anthracycline doxorubicin that undergoes β-glucuronidation, which partially detoxifies the dose-limiting side effects that are present with doxorubicin; equally cytotoxic to HeLa cells compared to doxorubicin (ID₅₀s = 9 μM).