Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze the breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides, such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.
Key Applications: Used as reporter gene; Enzymatic hydrolysis of glucuronides
Application Notes: β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means. β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Format: Supplied in 65% Saturated Ammonium Sulfate Solution
Source: Helix pomatia
Sulfatase Activity: ≤ 5000 u/mL