* Used to lyse living yeast cell walls to produce protoplast or spheroplast
* Can be used for glucan hydrolysis
Zymolyase-20T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is a lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate and contains 20,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product.
Key Applications: Yeast Cell Culture; Used to lyse cell wall of yeast
Application Notes: Zymolyase 20T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase has a low solubility. Use as a suspension.
Formulation: Lyophilized
pH: Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells; pH 6.5, 45 °C for hydrolysis of yeast glucan; pH Range: Stable through pH ranges 5-10
Source: Arthrobacter luteus
Specific Activity: 20,000 units/g
Specificity: The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwannimomyces and others.
Unit Definition: The amount of activity causing a decrease in absorbance at 800 nm of 30%.