Zymo-Seq WGBS Library Kit is the only kit available for whole genome bisulfite (WGBS) library preparation in a single tube. Zymo-Seq WGBS Library Kit incorporates tagmentation technology to eliminate the tedious fragmentation, enzymatic, and clean-up steps required by conventional, ligation-based library preparation methods. This streamlined workflow minimizes hands-on time, making it an ideal choice for higher throughput applications. To prepare Zymo-Seq WGBS libraries, intact genomic DNA is first bisulfite converted. Then, the following library preparation procedures are completed in a single tube: (1) second-strand synthesis, (2) adapterization via tagmentation, and (3) library amplification and indexing. After purification, libraries are ready for sequencing on Illumina instruments.
WGBS is the gold standard for DNA methylation studies as it provides base-level methylation quantification for all cytosines. Zymo-Seq WGBS Library Kit is ideal for any species for whole-genome methylation calling of CG, CHG, and CHH sites. Over 90% of human and mouse CpG sites can be detected from Zymo-Seq libraries.
- Whole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube.
- Reduced library preparation bias for accurate methylation calling
- Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples)
DNA Input
For optimal results, use 100 ng of high quality, intact genomic DNA as input. Protocol is compatible with inputs between 10 ng to 100 ng. DNA should be free of enzymatic inhibitors and RNA contamination. DNA can be resuspended in water, TE, or a low salt buffer.
Processing Time
~ 4 hours
Required Equipment
Microcentrifuge, thermal cycler with heated lid, magnetic separator, multichannel pipette (suggested)
Required material not provided
Illumina DNA Prep Kit (Illumina, Cat. No. 20018704 or 20018705)
Sequencing Platform
Libraries are compatible with all Illumina sequencing platforms (MiSeq, MiniSeq, HiSeq, NextSeq, NovaSeq)